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Specifications
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:XIAP
- Description:Rat XIAP ELISA kit
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Rat XIAP ELISA Kit exhibits high specificity and excellent specificity for the detection of rat XIAP. No significant cross-reactivity or interference between XIAP and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:0,156 - 10 ng/ml
- Storage temperature:4 °C
- Sensitivity:0,094 ng/ml
- Regulatory status:RUO
Specifications
About this item
Rat XIAP ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat XIAP in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Rat XIAP ELISA kit (A303836) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat XIAP in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for XIAP has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the XIAP present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-XIAP Antibody, which binds the captured XIAP present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of XIAP captured in each well. The concentration of XIAP can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.