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- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Rat
- Target protein:Myosin Light Chain Kinase/MLCK
- Description:Rat myosin light chain kinase/MLCK ELISA kit
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Rat Myosin Light Chain Kinase/MLCK ELISA Kit exhibits high specificity and excellent specificity for the detection of rat Myosin Light Chain Kinase/MLCK. No significant cross-reactivity or interference between Myosin Light Chain Kinase/MLCK and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:31,25 - 2000 pg/ml
- Storage temperature:4 °C
- Sensitivity:18,75 pg/ml
- Regulatory status:RUO
Rat Myosin Light Chain Kinase/MLCK ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Myosin Light Chain Kinase/MLCK in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Rat Myosin Light Chain Kinase/MLCK ELISA kit (A303704) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Myosin Light Chain Kinase/MLCK in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Myosin Light Chain Kinase/MLCK has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Myosin Light Chain Kinase/MLCK present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Myosin Light Chain Kinase/MLCK Antibody, which binds the captured Myosin Light Chain Kinase/MLCK present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Myosin Light Chain Kinase/MLCK captured in each well. The concentration of Myosin Light Chain Kinase/MLCK can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.