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- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Competitive
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:HSD17B3
- Description:Mouse HSD17B3 ELISA kit
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Mouse HSD17B3 ELISA kit exhibits high specificity and excellent specificity for the detection of mouse HSD17B3. No significant cross-reactivity or interference between HSD17B3 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:31,25 - 2000 pg/ml
- Storage temperature:4 °C
- Sensitivity:18,75 pg/ml
- Regulatory status:RUO
Mouse HSD17B3 ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of mouse HSD17B3 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 31,25 to 2000 pg/ml
- Sensitivity: 18,75 pg/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Mouse HSD17B3 ELISA kit (A303599) employs the competitive enzyme immunoassay technique for the quantitative measurement of mouse HSD17B3 in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with HSD17B3 antigen. During the incubation, HSD17B3 present in the samples or standards competes with the fixed amount of immobilised HSD17B3 for binding sites on the Biotinylated Anti-HSD17B3 antibody. The more HSD17B3 present in a sample or standard, the less Biotinylated Anti-HSD17B3 antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-HSD17B3 antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of HSD17B3 present in each sample or standard. The concentration of HSD17B3 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.