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Specifications
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:CIRP
- Description:Mouse CIRP ELISA kit
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Mouse CIRP ELISA Kit exhibits high specificity and excellent specificity for the detection of mouse CIRP. No significant cross-reactivity or interference between CIRP and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:15,625 - 1000 pg/ml
- Storage temperature:4 °C
- Sensitivity:9,375 pg/ml
- Regulatory status:RUO
Specifications
About this item
Mouse CIRP ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse CIRP in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection Range: 15,625 to 1000 pg/ml
- Sensitivity: 9,375 pg/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra to Assay: CV <8%, Inter to Assay: CV <10%
Mouse CIRP ELISA kit (A303577) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse CIRP in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for CIRP has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CIRP present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated anti-CIRP antibody, which binds the captured CIRP present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of CIRP captured in each well. The concentration of CIRP can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.