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Specifications
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:Eph Receptor B4/HTK
- Description:Mouse Eph receptor B4/HTK ELISA kit
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Mouse Eph Receptor B4/HTK ELISA kit exhibits high specificity and excellent specificity for the detection of mouse Eph Receptor B4/HTK. No significant cross-reactivity or interference between Eph Receptor B4/HTK and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:31,25 - 2000 pg/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:18,75 pg/ml
- Regulatory status:RUO
Specifications
About this item
Mouse Eph Receptor B4/HTK ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Eph Receptor B4/HTK in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 31,25 to 2000 pg/ml
- Sensitivity: 18,75 pg/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Mouse Eph Receptor B4/HTK ELISA kit (A76489) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Eph Receptor B4/HTK in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Eph Receptor B4/HTK has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Eph Receptor B4/HTK present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Eph Receptor B4/HTK Antibody, which binds the captured Eph Receptor B4/HTK present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Eph Receptor B4/HTK captured in each well. The concentration of Eph Receptor B4/HTK can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.