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Specifications
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:alpha Defensin 1
- Description:Mouse alpha defensin 1 ELISA kit
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Mouse alpha Defensin 1 ELISA kit exhibits high specificity and excellent specificity for the detection of mouse alpha Defensin 1. No significant cross-reactivity or interference between alpha Defensin 1 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:0,391 - 25 ng/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:0,234 ng/ml
- Regulatory status:RUO
Specifications
About this item
Mouse alpha Defensin 1 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse alpha Defensin 1 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 0,391 to 25 ng/ml
- Sensitivity: 0,234 ng/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Mouse alpha Defensin 1 ELISA kit (A76429) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse alpha Defensin 1 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for alpha Defensin 1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the alpha Defensin 1 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-alpha Defensin 1 Antibody, which binds the captured alpha Defensin 1 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of alpha Defensin 1 captured in each well. The concentration of alpha Defensin 1 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.