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Specifications
- Antibody type:Primary
- Antigen name:B-cell CLL/lymphoma 2
- Antigen symbol:BCL2
- Clonality:Monoclonal
- Clone:SPM530
- Conjugation:Unconjugated
- Flow cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG1 kappa
- Western blot:Yes
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P10415
- Antigen synonyms:BCL2
- Storage buffer:PBS with 0,1 mg/ml BSA and 0,05% sodium azide
- Storage temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0,2 mg/ml
- Shipping temperature:4 °C
- Immunogen:Amino acids 41-54 (GAAPAPGIFSSQPG) from the human protein were used as the immunogen for this anti-Bcl-2 antibody.
- Purification:Protein G affinity chromatography
- Size:100 µg
- Pk:100 µG
Specifications
About this item
This antibody recognises a protein of 25 to 26 kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Flow Cytometry: 0,5-1 µg/million cells in 0,1 ml Immunofluorescence: 0,5 - 1 µg/ml Western blot: 0,5 - 1 µg/ml Immunohistochemistry (FFPE): 0,5 - 1 µg/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the anti-Bcl-2 antibody for each application should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1mM EDTA, pH 9.0, for 10 - 20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimised for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Type: Primary
Antigen: BCL2
Clonality: Monoclonal
Clone: SPM530
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human,