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Specifications
- Antibody type:Primary
- Antigen name:tumor necrosis factor
- Antigen symbol:TNF
- Clonality:Monoclonal
- Clone:TMDa1-1
- Conjugation:Unconjugated
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgM kappa
- Reactivity:Human,Rat,Mouse
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P01375
- Antigen synonyms:TNF
- Storage buffer:PBS with 0,1 mg/ml BSA and 0,05% sodium azide
- Storage temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0,2 mg/ml
- Shipping temperature:4 °C
- Immunogen:Full length human recombinant protein was used as the immunogen for the TNF alpha antibody.
- Purification:PEG precipitation
- Size:100 µg
- Pk:100 µG
Specifications
About this item
Tumor Necrosis Factor Alpha (TNF alpha) is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. [UniProt].
Immunofluorescence: 1 - 2 µg/ml Immunohistochemistry (FFPE): 2-4 µg/ml for 30 min at RT (1) Titering of the TNF alpha antibody may be required for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1mM EDTA, pH 9.0, for 10 - 20 min followed by cooling at RT for 20 min.
Type: Primary
Antigen: TNF
Clonality: Monoclonal
Clone: TMDa1-1
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgM, kappa
Reactivity: Human, Mouse, Rat