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- Antibody type:Primary
- Antigen name:keratin 1, keratin 3, keratin 4, keratin 5, keratin 6A, keratin 8, keratin 10, keratin 14, keratin 15, keratin 16, keratin 19
- Antigen symbol:KRT1, KRT3, KRT4, KRT5, KRT6A, KRT8, KRT10, KRT14, KRT15, KRT16, KRT19
- Clonality:Polyclonal
- Conjugation:Unconjugated
- Flow cytometry:Yes
- Host:Rabbit
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG
- Reactivity:Human
- Western blot:Yes
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P04264, P12035, P19013, P13647, P48668, P05787, P13645, P02533, P19012, P08779, P08727
- Antigen synonyms:KRT1, KRT3, KRT4, KRT5, KRT6A, KRT8, KRT10, KRT14, KRT15, KRT16, KRT19
- Storage buffer:PBS with 0,1 mg/ml BSA and 0,05% sodium azide
- Storage temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0,2 mg/ml
- Shipping temperature:4 °C
- Immunogen:Recombinant full-length human KRT76 and KRT77 proteins were used as the immunogen for this pan Cytokeratin antibody.
- Purification:Protein A affinity chromatography
- Size:100 µg
- Pk:100 µG
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody detects acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins: 67 kDa (CK1); 64 kDa (CK3); 59 kDa (CK4); 58 kDa (CK5); 56 kDa (CK6); 52 kDa (CK8); 56.5 kDa (CK10); 50 kDa (CK14); 50 kDa (CK15); 48 kDa (CK16); 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. It is a broad spectrum anti pan-cytokeratin antibody, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It may be useful to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has high sensitivity in the recognition of epithelial cells and carcinomas.
Flow Cytometry: 0,5 - 1 µg/10e6 cells in 0,1 ml IF: 1 - 2 µg/ml WB: 0,5 - 1 µg/ml IHC (FFPE): 0,25-0,5 µg/ml for 30 min at RT Prediluted format: incubate for 30 min at RT (1) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the pan Cytokeratin antibody to be titered up or down for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimised for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Type: Primary
Antigen: KRT1, KRT3, KRT4, KRT5, KRT6A, KRT8, KRT10, KRT14, KRT15, KRT16, KRT19
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype: IgG
Reactivity: Human