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Specifications
- Antibody type:Primary
- Antigen name:tyrosyl-DNA phosphodiesterase 2
- Antigen symbol:TDP2
- Clonality:Monoclonal
- Clone:TDP2/1258
- Conjugation:Unconjugated
- Flow cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG2b kappa
- Reactivity:Human
- Western blot:Yes
- Format:Purified Antibody
- Form:Liquid
- Gene ID:O95551
- Antigen synonyms:TDP2
- Storage buffer:PBS with 0,1 mg/ml BSA and 0,05% sodium azide
- Storage temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0,2 mg/ml
- Shipping temperature:4 °C
- Immunogen:Human recombinant full length protein was used as the immunogen for this TDP2 antibody.
- Purification:Protein G affinity chromatography
- Size:100 µg
- Pk:100 µG
Specifications
About this item
This mAb recognises a protein of 41 kDa, which is identified as TDP2, or ETS1 associated protein II. It is a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. catalyses the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.
Flow Cytometry: 0,5 - 1 µg/10^6 cells in 0,1 ml IF: 0,5 - 1 µg/ml IHC (FFPE): 0,5 - 1 µg/ml for 30 min at RT The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TDP2 antibody to be titered up or down for optimal performance.
Type: Primary
Antigen: TDP2
Clonality: Monoclonal
Clone: TDP2/1258
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2b, kappa
Reactivity: Human