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- Antibody type:Primary
- Antigen name:Double Stranded DNA Antibody [SPM603]
- Antigen symbol:dsDNA
- Clonality:Monoclonal
- Clone:SPM603
- Conjugation:Unconjugated
- Flow cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG3 kappa
- Reactivity:Human
- Format:Purified Antibody
- Form:Liquid
- Storage buffer:PBS with 0,1 mg/ml BSA and 0,05% sodium azide
- Storage temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0,2 mg/ml
- Shipping temperature:4 °C
- Immunogen:Nuclei of Burkitt's cells were used as the immunogen for the Double Stranded DNA antibody.
- Tested applications:Immunocytochemistry
- Purification:Protein G affinity chromatography
- Size:100 µg
- Pk:100 µG
Marker antibodies are useful in identification of organelles in cells, tissues, and biochemical preparations. This mAb recognises the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. Double stranded (ds) DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.
Flow Cytometry: 0,5-1 µg/million cells in 0,1 ml Immunofluorescence: 1 - 2 µg/ml Immunocytochemistry (Acetone-fixed): 0,5 - 1 µg/ml for 30 min at RT Immunohistochemistry (FFPE): 1 - 2 µg/ml for 30 min at RT (1) Optimal dilution of the Double Stranded DNA antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6,0, for 10 - 20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimised for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Type: Primary
Antigen: Double Stranded DNA Antibody [SPM603]
Clonality: Monoclonal
Clone: SPM603
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG3, kappa
Reactivity: Human