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Specifications
- Antibody type:Primary
- Antigen name:mucin 3A, cell surface associated
- Antigen symbol:MUC3A
- Clonality:Monoclonal
- Clone:MUC3/1154
- Conjugation:Unconjugated
- Flow cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG2a kappa
- Reactivity:Human
- Format:Purified Antibody
- Form:Liquid
- Gene ID:Q02505
- Antigen synonyms:MUC3A
- Storage buffer:PBS with 0,1 mg/ml BSA and 0,05% sodium azide
- Storage temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0,2 mg/ml
- Shipping temperature:4 °C
- Immunogen:Amino acids (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS) which contains two of the MUC3 tandem repeats was used as the immunogen for the MUC3 antibody.
- Purification:Protein G affinity chromatography
- Size:100 µg
- Pk:100 µG
Specifications
About this item
It recognises a protein of HMW, identified as mucin 3 glycoprotein (MUC3). This mAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3). The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.
Flow Cytometry: 0,5-1 µg/million cells in 0,1 ml Immunofluorescence: 1 - 2 µg/ml Immunohistochemistry (FFPE): 0,5 - 1 µg/ml for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) Optimal dilution of the MUC3 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6,0, for 10 - 20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimised for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Type: Primary
Antigen: MUC3A
Clonality: Monoclonal
Clone: MUC3/1154
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG2a, kappa
Reactivity: Human