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- Antibody type:Primary
- Antigen name:calponin 1, basic, smooth muscle
- Antigen symbol:CNN1
- Clonality:Monoclonal
- Clone:CALP
- Conjugation:Unconjugated
- Flow cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG1 kappa
- Reactivity:Human,Rat
- Western blot:Yes
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P51911
- Antigen synonyms:CNN1
- Storage buffer:PBS with 0,1 mg/ml BSA and 0,05% sodium azide
- Storage temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0,2 mg/ml
- Shipping temperature:4 °C
- Immunogen:Crude human uterus extract was used as the immunogen for the Calponin antibody.
- Purification:Protein G affinity chromatography
- Size:100 µg
- Pk:100 µG
Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this mAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
Flow Cytometry: 0,5-1 µg/million cells in 0,1 ml Immunofluorescence: 1 - 2 µg/ml Immunohistochemistry (FFPE): 0,5 - 1 µg/ml for 30 min at RT (1) Optimal dilution of the Calponin antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1mM EDTA, pH 9.0, for 10 - 20 min followed by cooling at RT for 20 min.
Type: Primary
Antigen: CNN1
Clonality: Monoclonal
Clone: CALP
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human, Rat