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Specifications
- Antibody type:Primary
- Antigen name:trefoil factor 1
- Antigen symbol:TFF1
- Clonality:Monoclonal
- Clone:GE2
- Conjugation:Unconjugated
- Flow cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG1 kappa
- Reactivity:Cynomolgus monkey,Human
- Western blot:Yes
- Format:Purified Antibody
- Form:Liquid
- Gene ID:P04155
- Antigen synonyms:TFF1
- Storage buffer:PBS with 0,1 mg/ml BSA and 0,05% sodium azide
- Storage temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0,2 mg/ml
- Shipping temperature:4 °C
- Immunogen:Amino acids CFDDTVRGVPWCFYPNTIDVPPEEECEF (57-84) from the C-terminus of human Estrogen inducible protein pS2 was used as the immunogen for this antibody.
- Purification:Protein G affinity chromatography
- Size:100 µg
- Pk:100 µG
Specifications
About this item
This antibody recognises a polypeptide of ~6.5 kDa, identified as Estrogen Inducible Protein pS2. The antibody's epitope is localized between amino acids 57-84. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain. Estrogen Inducible Protein pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60% of breast carcinomas are positive for estrogen inducible protein pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. It is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that estrogen inducible protein pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.
Flow Cytometry: 0,5-1 µg/million cells IF: 0,5 - 1 µg/ml IHC (FFPE): 0,5-1,0 µg/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of FFPE tissues REQUIRES digestion with Pronase E at 1mg/ml in PBS for 15 min at 37°C. Heat Induced Epitope Retrieval is not suitable for this antibody.
2. The prediluted format is supplied in a dropper bottle and is optimised for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Type: Primary
Antigen: TFF1
Clonality: Monoclonal
Clone: GE2
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human, Cynomolgus Monkey