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Specifications
- Antibody type:Primary
- Antigen name:X-ray repair complementing defective repair in Chinese hamster cells 6
- Antigen symbol:XRCC6
- Clonality:Monoclonal
- Clone:KU729
- Conjugation:Unconjugated
- Flow cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG1 kappa
- Format:Purified Antibody
- Form:Liquid
- Gene ID:Q6FG89
- Antigen synonyms:XRCC6
- Storage buffer:PBS with 0,1 mg/ml BSA and 0,05% sodium azide
- Storage temperature:Aliquot and Store at 2…8 °C. Avoid freez-thaw cycles.
- Concentration:0,2 mg/ml
- Shipping temperature:4 °C
- Immunogen:Nuclear extract of human HL-60 cells was used as the immunogen for this Ku70 + Ku80 antibody.
- Tested applications:Immunocytochemistry
- Purification:Protein G purified monoclonal antibody
- Size:100 µg
- Pk:100 µG
Specifications
About this item
This antibody recognises a dimer of two proteins of 70 kDa (Ku70) and ~80 kDa (Ku80), identified as two subunits of Ku. Antbody KU729 recognises a conformational epitope of the Ku70 + Ku80 dimer, which is destroyed during Western blotting. The Ku70 + Ku80 dimer is important for function of a 460 kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Flow cytometry: 0,5 - 1 µg/10e6 cells IF: 0,5-1,0 µg/ml Immunocytochemistry (Acetone-fixed cells): 0,25-0,5 µg/ml for 30 minutes at RT The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ku70 + Ku80 antibody to be titered up or down for optimal performance.
Type: Primary
Antigen: XRCC6
Clonality: Monoclonal
Clone: KU729
Conjugation: Unconjugated
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human, Primates