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Specifications
- Cell Format:Cryopreserved
- Pk:0,1 mg
- Description:Normal; Source: Mouse; Tissue: Fetal
- Storage conditions:Store at 2...8 °C for continuous use. For extended storage, freeze working aliquots at −70 °C.
- Storage buffer:Fetus lysate (14 Day Fetus) is supplied in SDS sample buffer containing 5% β-mercaptoethanol
- Concentration:2 mg/ml
- Tested applications:WB
Specifications
About this item
Fetus lysate (14 Day Fetus) was prepared by homogenisation in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7,4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0,1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin.
Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6,8, 12,5% glycerol, 1% sodium dodecylsulfate, 0,01% bromophenol blue) containing 5% β-mercaptoethanol.