Order Entry
Northern Ireland
ContactUsLinkComponent
SPINeasy® Plasmid Purification, MidiPrep Kit, MP Biomedicals
SPINeasy® Plasmid Purification, MidiPrep Kit, MP Biomedicals
Catalog # 116539025.
Supplier:  MP Biomedicals
CAS Number:  
undefined
SPINeasy® Plasmid Purification, MidiPrep Kit, MP Biomedicals
Catalog # 116539025.
Supplier:  MP Biomedicals
CAS Number:  

Some Products May Appear Restricted

To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.

If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/us/en/login/register)

 

If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation;  additional documentation will be requested for these items prior to shipment. 

Specifications

  • Pk:
    1 KIT
  • Method / Format:
    Column (silica-based)
  • Prep size:
    Midi
  • Sample type:
    Bacterial culture
  • Target molecule:
    Plasmid
  • Description:
    SPINeasy plasmid MidiPrep kit, 25 tests
  • Storage temperature:
    Store at room Temperature (15...30 ℃), dry environment

Specifications

About this item

SPINeasy Plasmid Midiprep kit is a silica-membrane spin-column kit for the purification of high-copy and low-copy number plasmid DNA from 25 to 50 ml of transformed bacterial (E.coli) culture.

Using our specially formulated buffers, bacterial cells are disrupted by alkaline lysis to release plasmid DNA. Cell debris, such as chromosomal DNA and proteins, are precipitated by neutralization and cleared through centrifugation. Subsequently, plasmid DNA in the lysate supernatant is adsorbed on a silica membrane, washed and eluted in a convenient process using the spin column technology. Upto 1 mg of plasmid DNA can be purified through this simple workflow, which is immediately ready for routine molecular biology laboratory applications.

Key applications are In-vitro transcription, cloning, NGS, PCR, qPCR, restriction digestion, transformation, genomics, molecular biology.

Certifications: Hazard Class 8
Delivery: Kit includes 60 ml Resuspension Buffer SD1, 60 ml Alkaline Lysis Buffer SD2, 90 ml Neutralization Buffer SD3, 70 ml Wash Buffer DW1, 12 ml Wash Buffer DW2, 30 ml Elution Buffer SD, 600 µl RNase A, 25× Column SD with collection tubes.