Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane the method allows the precise analysis and identification of DNA bands on a gel. Using this kit, students use Southern blotting to find a point mutation in the haemoglobin gene indicating Sickle Cell Anaemia.

• For five lab groups
• Electrophoresis, 45 minutes; blotting, overnight; staining and destaining, 10 minutes

Also required: Electrophoresis tank, power supply, water bath and 80 °C incubator.