Kits: restriction enzyme cleavage patterns of DNA, Ready-to-Load™

Kits: restriction enzyme cleavage patterns of DNA, Ready-to-Load™

Plasmid and lambda DNA are pre-digested with restriction endonucleases that recognise and cut double-stranded DNA within or near defined base sequences. Digests are separated by agarose gel electrophoresis.

For six lab groups

Also required: Electrophoresis tank, power supply, micropipettes (5 to 50 µl adjustable or 40 µl fixed volume) with tips, and a white light box is recommended.

: Kit includes instructions, Ready-to-Load™ DNA samples, agarose powder, practice gel loading solution, electrophoresis buffer, InstaStain® DNA stain, 1 ml calibrated drop pipette, 100 ml graduated cyclinder and microtipped transfer pipettes.

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Water, HiPerSolv CHROMANORM® for HPLC

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Methanol ≥99.8%, HiPerSolv CHROMANORM® Reag. Ph. Eur., gradient grade for HPLC

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Acetonitrile ≥99.9%, LiChrosolv® Reag. Ph. Eur., gradient grade for liquid chromatography, Supelco®

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VWR®, Nitrile Gloves

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pH indicator strips, MQuant®, Supelco®

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VWR® Facial Tissues

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Acetonitrile ≥99.9%, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments

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Acetone ≥99%, TECHNICAL

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Sealing film for manual application, Parafilm® M

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VWR® NITRILE LIGHT, Nitrile Gloves

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VWR®, Transfer Pipettes, High Performance

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Acetonitrile, anhydrous (max. 0.003% H₂O) ≥99.95%, HiPerSolv CHROMANORM® Reag. USP, Reag. Ph. Eur., ACS, super gradient grade suitable for UPLC/UHPLC instruments

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VWR® Serological Pipette, Standard Line

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Nitrile gloves, Kimtech™ Purple Nitrile™

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Sodium hydroxide 0.1 mol/l (0.1 N) in aqueous solution, AVS TITRINORM Reag. Ph. Eur. volumetric solution

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