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Specifications
- Antibody type:Primary
- Antigen name:MBD1
- Clonality:Polyclonal
- Conjugation:Alexa Fluor® 750
- Host:Rabbit
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG
- Reactivity:Human,Rat
- Western blot:Yes
- Epitope:101-200/605
- Form:Liquid
- Gene ID:4152
- Antigen synonyms:CXXC3|Methyl CpG binding protein splice variant 2|Methyl CpG binding protein splice variant 4|PCM1|MBD 1|Regulator of fibroblast growth factor 2 FGF 2 transcription|The regulator of fibroblast growth factor 2FGF 2 transcription|RFT|Methyl CpG binding domain protein 1 isoform PCM1|Protein containing methyl CpG binding domain 1|MBD1|CXXC 3|Methyl CpG binding domain protein 1|MECP1 COMPLEX|Methyl CpG binding protein MBD1|Methyl CpG binding protein splice variant 3|PCM 1|Methyl CpG binding protein splice variant 1
- Modification:Unmodified
- Storage buffer:Aqueous buffered solution containing 0,01 M TBS (pH 7,4) with 1% BSA, 0,03% Proclin300 and 50% Glycerol
- Molecular weight:60 kDa
- Storage temperature:Store at ‒20 °C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Concentration:1 µg/µl
- Shipping temperature:4 °C
- Immunogen:KLH conjugated synthetic peptide derived from human MBD1
- Tested applications:ICC
- Purification:Purified by Protein A
- Pack type:Vial
- Pk:100 µl
Specifications
About this item
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalise with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.
Type: Primary
Antigen: MBD1
Clonality: Polyclonal
Clone:
Conjugation: ALEXA FLUOR® 750
Public Immunogen Range: 101-200/605
Host: Rabbit
Isotype: IgG
Reactivity: Human; Rat