Some Products May Appear Restricted
To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.
If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/us/en/login/register)
If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation; additional documentation will be requested for these items prior to shipment.
Specifications
- Pk:96 Tests
- Assay Type:Sandwich
- Primary antibody reactivity:Mouse
- Description:Troponin I ELISA kit
Specifications
About this item
A sandwich ELISA kit for in vitro quantitative determination of mouse troponin I concentrations in serum, plasma and other biological fluids.
- Detection Range: 31,25 to 2000 pg/ml
- Coefficient of variation is <10%
- Sensitivity: 18,75 pg/ml
- Specificity: no significant cross-reactivity or interference between mouse Tn-I and analogues was observed
Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Troponin I ELISA kit uses the sandwich-ELISA principle. The micro ELISA plate provided in the kit has been pre-coated with an antibody specific to mouse troponin I. Standards or samples were added to the micro ELISA plate that reacts with biotinylated detection antibody specific for mouse troponin I and Avidin-Horseradish Peroxidase (HRP) conjugate which on the addition of substrate form blue color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of human troponin I. The concentration of mouse troponin I in the samples can be calculated by comparing the OD of the samples to the standard curve.