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Specifications
- Pk:1 kt
- Description:PCR polishing kit
- Details:Optimises blunt-end cloning efficiencies of PCR-generated fragments, generates blunt-ended DNA fragments in 30 minutes. Sufficient for 40 reactions.
Specifications
About this item
PCR polishing kit increases blunt-end cloning efficiencies associated with PCR-generated fragments.
- Generates blunt-ended DNA fragments in 30 minutes
- More efficient than Klenow polishing
- Optimizes blunt-end cloning efficiencies
PCR Polishing Kit enables an increase in blunt-ended cloning efficiencies associated with polymerase chain reaction (PCR)-generated fragments. It polishes the ends of the 3’ overhang extensions of polymerase-generated DNA fragments directly from PCR amplification and can completely fill in 5’ overhangs to generate blunt ends.
Dramatic increases in the population of blunt-end DNA fragments following polishing with Agilent’s PCR Polishing Kit enables significant increase in overall experimental efficiency associated with procedures utilising blunt-ended ligation.