Some antibodies are difficult to remove from Western blots and require Longer incubation times or incubation temperatures greater than 22 °C. Restore™ PLUS Western Blot Stripping Buffer was developed to reduce incubation times while keeping incubations at room temperature. High-affinity antibodies can be quickly and effectively stripped from Western blots without removing transferred proteins, thereby allowing multiple reprobes of the target.

• Ready and easy to use - no dilution necessary, no offensive odours and room temperature storage
• Compatible with commonly used Western blotting reagents and other materials - use on nitrocellulose and PVDF membranes, stored wet or dry; works with blocking buffer, enzyme conjugate and chemiluminescent substrate of choice
• Cost effective - saves valuable time and sample; strip blots effectively the first time
• Robust, but gentle - transferred proteins remain viable; strip the same blot up to five times
• Flexible - strip and re-probe to optimise antibody concentrations; strip and re-probe for new antigen of interest