Phusion® U hot start PCR master mix is a novel engineered high fidelity enzyme developed using fusion technology.

• Accuracy: high fidelity DNA polymerase (25X Taq)
• Uracil-tolerance: engineered to incorporate dUTP and amplify uracil containing templates
• Specificity: hot start for reduced nonspecific amplification and primer degradation
• Speed: short extension times (15 to 30 s/kb)
• Store at –20 °C

Due to a proprietary mutation in the so called dUTP binding pocket of Phusion®, Phusion® U overcomes an important limitation of proofreading enzymes - it can incorporate dUTP and read through uracil present in DNA templates.

In addition to uracil usage possibility, it features all the superior properties of other Phusion® DNA polymerases - great accuracy, speed, ability to amplify long amplicons up to 20 kb and a high specificity with Affibody based hot start. These features make DNA Polymerase an excellent choice for such important applications as amplification of bisulphite converted or damaged DNA as well as use of carryover contamination control.

It is convenient 2X mix containing Phusion® U hot start DNA polymerase, nucleotides and optimised reaction buffer including MgCl₂. Only template and primers need to be added by the user.