HIF-1α transcription factor assay Kit
Catalog # CAYM10006910-96
Supplier: Cayman Chemical
CAS Number:
Specifications
- Pk:1 KIT
- Description:HIF-1α Transcription Factor Assay Kit, 96-wells
- Environmentally Preferable:
- Storage temperature:–80 °C
Specifications
About this item
HIF-1α Transcription Factor Assay Kit is a sensitive, non radioactive method for detecting HIF-1α from whole cell lysates.
- 96-well plate format replaces EMSAs
- Capture the transcription factor using a specific dsDNA sequence bound to the plate
- Detect the dsDNA-bound transcription factor with specific antibodies in an ELISA format
The HIF (hypoxia-inducible factor) transcription factor is a member of the basic-helix-loop-helix (bHLH) family of transcription factors and plays an important role in maintaining cellular oxygen homeostasis. HIF-1α has emerged as an important drug target in breast and prostate cancer, cardiovascular disease, and ischemia.
HIF-1αTranscription Factor Assay is a sensitive ELISA-based method for detecting HIF-1α DNA binding activity in nuclear extracts and whole cell lysates.
Delivery: Kit contains: Assay buffer, Reagent A, positive control, binding buffer, antibody, concentrate, polysorbate 20, competitor dsDNA, HRP conjugate, plate and cover, developing and stop solution.