About this item
Prepared by high salt extraction of HeLa nuclei (human cervical cancer cell line), this extract is a rich source of HDAC activity, useful for inhibitor screening or immunoprecipitation of HDAC containing complexes. Typically, 0,5 µl or less per well is sufficient for assay with the HDAC FLUOR DE LYS® fluorometric activity assay.
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Specifications
- Storage buffer:Liquid. In 0,1 M KCl, 20 mM HEPES/NaOH, pH 7,9, 20% (v/v) glycerol, 0,2 mM EDTA, 0,5 mM DTT, 0,5 mM PMSF.