100-reaction single-round RNA amplification and Biotin labeling system

About this item

Improved data quality and analysis results through greater biotin incorporation.

The Labeling System provides an optimized protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples (1 µg to 5 µg) in less than 24 hours for array analysis. While cDNA quantities produced are sufficient for gene expression, manual sample preparation involving multiple enzymatic reactions and product purification steps are time consuming and error-prone.

The 100-Reaction Single- Round RNA Amplification and Biotin Labeling System has been optimized for superior performance across several automated liquid handling platforms.The result is a comprehensive system that reduces variability while subsequently improving reproducibility, data quality and throughput for automated genomic environments.The complete system is composed of reagents for cDNA synthesis and in vitro transcription labeling and does not provide materials required for purification.

Delivery: Kit contains 400 µl dNTP Mix (dN), 100 µl Promoter Primer (P), 200 µl First Strand Buffer (FB), 800 µl DTT (D), 100 µL Reverse Transcriptase (RT), 100 µl RNase Inhibitor (I), 500 µl DNA Polymerase (DP), 100 µl RNase H (RH), 1,5 ml Second Strand Buffer (SB), 600 µl IVT Reaction Buffer (RB), 600 µl 10X Biotin-Labeled Ribonucleotides (B), 600 µl Enhancer Cocktail (EC), 300 µl T7 RNA Polymerase (T7) and 13 ml Nuclease-free Water (W).

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