AMPIGENE® qPCR 1-Step Probe Kit No-ROX

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

AMPIGENE® qPCR 1-Step Probe Kits use the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The modified MMLV RTase is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase. The RTase is thermostable and extremely active. AMPIGENE® qPCR 1-Step Probe Kits are designed for use on a variety of probes including: Taqma ®, Molecular Beacons®, and Scorpion probes®.An optimized buffer system allows efficient amplification and proprietary enhancers prevent the formation of primers/dimers to increase sensitivity and specificity. ROX is a reference dye which is used to normalize fluorescent signal in qPCR. AMPIGENE® qPCR 1-Step Probe Kits come with either Lo-ROX, Hi-ROX, or No-ROX concentration. For Lo-ROX and Hi-ROX, see Prod. Nos. ENZ-NUC110 and ENZ-NUC111. Choose the appropriate ROX concentration for the instrument.

: Contains 2x AMPIGENE® qPCR 1-Step Probe Mix No-ROX (includes dNTP, and polymerases) 20x RTase (with RNase inhibitor).

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Application:
qPCR probe
One step:
Yes