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Specifications
- Antibody type:Primary
- Antigen name:Glycogen Synthase Kinase 3 alpha
- Antigen symbol:GSK3A
- Clonality:Monoclonal
- Clone:6D3
- Host:Mouse
- Isotype:IgG1
- Reactivity:Human,Mouse
- Western blot:Yes
- Seq:02
- Form:Lyophilized from 1 ml of 2× PBS containing 0,09% sodium azide, PEG, and sucrose.
- Antigen synonyms:GSK-3 alpha|Glycogen synthase kinase-3 alpha|GSK3A|Serine/threonine-protein kinase GSK3A
- Purification:Thiophilic adsorption and size exclusion chromatography purified
- Pk:100 µG
Specifications
About this item
Glycogen Synthase Kinase 3β (GSK-3β) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3β on serine 9, which prevents GSK-3β from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3β is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3β. The phosphorylated serine binds to the GSK-3β priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3β participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3β is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3β via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3β with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3β inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.
- For Western Blot
Recognizes human and mouse GSK-3alpha/beta.
Reconstitute with 1ml water (15 minutes at room temperature).
Type: Primary
Antigen: GSK-3ALPHA
Clonality: Monoclonal
Clone: 6D3
Conjugation:
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: Human, Mouse