Specifications
- Pk:10 µG
- Protein/peptide type:Recombinant
- Source:HEK293 cells
- Species:Human
- Tag sequence:DDDDK Tag
- Storage conditions:Stable for at least 6 months after receipt when stored at −20 °C.
- Biological activity:Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).
- Protein synonyms:ACRP30headless:TNF-alpha|ACRP30headless:Tumor Necrosis Factor-alpha|ACRP30headless:TNFSF2
- Protein/peptide name:TNF alpha multimeric
- Purity:>95% (SDS-PAGE)
- Sequence:16-108
- Formulation:Lyophilized, Contains PBS.
Specifications
About this item
Tumour necrosis factor (TNF, cachexin or cachectin and formerly known as tumour necrosis factor-alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. MultimericTNF-alpha™ is a high activity construct in which two trimeric TNF-alpha ligands are artificially linked via the collagen domain of ACRP30. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-alpha, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-alpha (TNF-alpha (human) (multimeric) (rec. ) (Prod. No. AG-40B-0019). Since the MultimericTNF-alpha™ mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-alpha protein that can activate the TNF-R2. For TNF-R1 activation, either "normal" TNF-alpha or MultimericTNF-alpha™ can be used.
Fusion-Tag: DDDDK Tag
This recombinant proteins is for research use only.