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Specifications
- Antigen name:Mitogen-Activated Protein Kinase 1
- Antigen symbol:MAPK1
- Clonality:Polyclonal
- Conjugation:Unconjugated
- Host:Rabbit
- ImmunoChemistry:Yes
- Reactivity:Xenopus,Human,Rat,Mouse
- Western blot:Yes
- Form:Liquid
- Antigen synonyms:P42MAPK|PRKM2|ERK2|PRKM1|MAPK2|ERK|ERT1|p38|p41mapk|ERK-2|p40|p42-MAPK|p41
- Immunogen:ERK/MAPK (Thr202/Tyr204) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Thr202 and Tyr204 of ERK/MAPK.
- Size:100 µl
- Pk:100 µl
Specifications
About this item
Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204.
Immunolabeling in UV treated Jurkat Cells or in a rat brain lysate is blocked by the Thr202/Tyr204 phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Human, mouse, rat and Xenopus have 100% amino acid sequence identity with the antigen used to raise the antibody. Dot blots and Western blots with a rat brain lysate were performed with each lot. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Affinity Purified
100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Aliquot and store at -80°C or below. Avoid multiple freeze-thaw cycles.
Type:
Antigen: MAPK1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human, Mouse, Rat, Xenopus