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Specifications
- Antigen name:Raf-1 Proto-Oncogene, Serine/Threonine Kinase
- Antigen symbol:RAF1
- Clonality:Polyclonal
- Conjugation:Unconjugated
- Host:Rabbit
- Reactivity:Xenopus,Chicken,Human,Bovine,Dog,Rat,Mouse
- Western blot:Yes
- Form:Liquid
- Antigen synonyms:Raf-1|CRAF|RAF|NS5|cRaf|C-raf|c-Raf|Proto-oncogene c-RAF
- Immunogen:Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser301 of rat Raf-1.
- Size:100 µl
- Pk:100 µl
Specifications
About this item
The Ras pathway is a critical signal transduction cascade involved in regulating cellular proliferation, differentiation, survival, and oncogenic transformation. Members of the Raf serine/threonine kinase family are key intermediates in this cascade, functioning to relay signals from activated Ras to the downstream protein kinases MEK and ERK (Marshall, 1996). Previous studies have shown that phosphorylation is required for Raf-1 activation (Dhillon and Kolch, 2002; Chong et al., 2003). Recent work has demonstrated that phosphorylation also regulates the downregulation of Raf (Dougherty et al., 2005) with two sites participating: Ser301 and Ser642.
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Affinity Purified
100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Type:
Antigen: RAF1
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: Human, Mouse, Rat, Dog, Bovine, Chicken, Xenopus