Lysis buffer, CTAB

Cationic detergent cetyltrimethylammonium bromide (CTAB) is used to liberate and complex with total cellular nucleic acids.

Form: Liquid
Storage temperature: Ambient
Specification pH (20 °C): 8,0 ±0,1

This buffer contains 2% w/v CTAB, 20 mM EDTA.Na₂.2H₂O, 1,4 M sodium chloride and 100 mM tris ultrapure. Effective for removing polysaccharides, phenolic compounds and other enzyme-inhibiting contaminants found in plant cells in the supernatant.

Environmentally Preferable:

Frequently Bought Together

Trypsin

: Biowest

Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) ≥99.9%, Ultrapure

: VWR Chemicals

Cetrimonium chloride 50% in 2-propanol 60% (v/v)/water 40% (v/v) for synthesis, Sigma-Aldrich®

: Merck

Ethanol for molecular biology, Sigma-Aldrich®

: Merck

Macrosep® Advance Centrifugal Devices

: Cytiva (Formerly Pall Lab)

pH indicator papers, MQuant®, Supelco®

: Merck

Sampler, Vampire

: BURKLE

Ethanol absolute, Uvasol® for spectroscopy, Supelco®

: Merck

Peqlab peqGOLD, DNA Ladder, 1 kb

: VWR Chemicals

Chloroform : Isoamyl alcohol (24:1) (v/v)

: PanReac AppliChem

D-(+)-Biotin, Sigma-Aldrich®

: Merck Millipore (Calbiochem‎)

Pallflex® filters

: Cytiva (Formerly Pall Lab)

VWR®, Bottles, Wide Neck, Square, with Screw Cap

: VWR Collection

2-Propanol 99.5% for molecular biology DNase-, RNase-, protease-free

: Thermo Fisher Scientific

Research buffers and reagents

: G-Biosciences

Phusion® Green High-Fidelity DNA Polymerase

: FINNZYMES REAGENTS

Microcentrifuge tubes, 0,5/0,7/1,5/2,0/2,2 ml

: Greiner Bio-One