Anti-alpha GalCer:CD1d Complex Mouse Monoclonal Antibody (PE (Phycoerythrin)) [clone: L363]

About this item

Description: This L363 monoclonal antibody reacts with alpha-galactosylceramide bound to the mouse MHC class I-like protein CD1d. This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Valpha14Jalpha18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation. The L363 antibody has been reported to neutralize iNKT cell activation by alpha-galactosylceramide:CD1d complexes. Applications Reported: This L363 antibody has been reported for use in flow cytometric analysis. Applications Tested: This L363 antibody has been tested by flow cytometric analysis of alpha-galactosylceramide-loaded mouse CD1d-transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser This complex is recognized by invariant natural killer T (iNKT) cells, a subset of T lymphocytes that expresses the invariant TCR alpha chain Va14Ja18. Activation of iNKT cells leads to production of Th1 and Th2-associated cytokines as well as dendritic cell maturation.

Flow Cytometry: 0.25 µg/test

Type: Primary
Antigen: alpha GalCer:CD1d Complex
Clonality: Monoclonal
Clone: L363
Conjugation: PE (Phycoerythrin)
Epitope:
Host: Mouse
Isotype: IgG2a, kappa
Reactivity: Mouse

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Specifications

Catalog No:
BOSSBS-3270R
EBIO12-2019-82

Antigen symbol:
MAP2K1 + MAP2K2
alpha GalCer:CD1d Complex

Antigen name:
Mitogen-activated protein kinase Kinase 1 And 2
alpha GalCer:CD1d Complex

Antigen synonyms:
PRKMK2|MAPKK 2|MAPKK 1|MAP kinase kinase 2|MAPK/ERK kinase 2|Map2k2|dual specificity mitogen-activated protein kinase kinase 1|MAP2K1|MEK 1|ERK activator kinase 2|ERK activator kinase 1|MEK2|MKK2|Dual specificity mitogen-activated protein kinase kinase 2|MAP kinase kinase 1|MAPK/ERK kinase 1|mitogen-activated kinase 1

Antibody type:
Primary
Primary

Clonality:
Polyclonal
Monoclonal

Conjugation:
Unconjugated
PE (Phycoerythrin)

Clone:
L363

Reactivity:
Human, Rat, Mouse
Mouse

Host:
Rabbit
Mouse

Gene ID:
5604,5605
12479

Isotype:
IgG
IgG2a kappa

Western blot:
Yes

ImmunoChemistry:
Yes

Flow cytometry:
Yes

Modification:
phospho-Ser218/Ser222

Immunogen:
180-230/393

Form:
Liquid
Liquid

Purification:
Purified by Protein A
Affinity chromatography

Concentration:
1 ug/ul
0.2 mg/mL

Cross adsorption:
No

Storage buffer:
Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0,09% sodium azide. Store at -20°C for 12 months.
PBS, 0.1% gelatin, 0.09% sodium azide, pH 7.2