Some Products May Appear Restricted
To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.
If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/us/en/login/register)
If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation; additional documentation will be requested for these items prior to shipment.
Specifications
- Pk:25 AU
- Enzyme type:Recombinant
- Species:Human
- Seq:01
- Enzyme name:Caspase 1
Specifications
About this item
Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.
The recombinant human caspases 1 - 3 and 6 - 10 are members of the ICE (interleukin-1 β converting enzyme) family of cysteine proteases. They exist in cells as inactive pro-enzymes. During apoptosis, pro-caspases 1 - 3 and 6 - 10 are processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The matured (processed) form of these caspases consists of large and small subunits, which associate to form a heterotetramer active enzyme.
The active recombinant caspase-2 cleaves caspase-2 chromogenic substrate Ac-VDVAD-pNA, and the signal can be detected spectrophotometrically.
The active caspase-6 and caspase-7 have been shown in the involvement of the proteolysis of poly (ADP-ribose) polymerase (PARP), an enzyme that is involved in DNA repair and genomic maintenance. The active recombinant caspase-6 cleaves the substrates consisting of consensus sequence VEID (e.g. VEID-AFC and VEID-pNA).