The FastRNA™ SPIN Kit for Yeast quickly and efficiently isolates high-quality, total RNA from tough-to-lyse yeast strains, fungi, and algae in approximately 15 minutes using a specialised Lysing Matrix (Yttria-stabilised zirconium oxide, 0,5 mm) for cell lysis and SPIN columns for the purification process.

The FastRNA™ SPIN Kit isolates both large and small RNA species without the use of phenol or reducing agents. Small RNAs (eg. tRNAs, microRNAs) can be recovered following a simple adjustment within the RNA isolation protocol. RNA (~5 µg) isolated from 10¹ to 10⁵ cells can be eluted into volumes as little as 6 µl in less than 15 minutes. High quality total RNA (A260/A280 >1,8, A260/A230 >1,8) is recovered. RNA is eluted with RNase-free water and can be stored at –70 °C. The addition of RNase inhibitors is optional, but highly recommended for prolonged storage.

For research purposes only.