Glycoprotein Isolation Kit, Cona And WGA, Pierce™

The glycoprotein isolation kit allows isolation of glycoproteins from complex mixtures, including serum, tissue and cultured cell lysates. The procedure uses concanavilin A (ConA) or wheat germ agglutinin (WGA) as a ligand to bind a specific class of glycoprotein from complex protein samples - those containing alpha-lined mannose or terminal glucose residues (in case of ConA) and N-Acetyl glucosamine (GlcNAc) groups or sialic acid (in case of WGA). This kit has been used to isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes.

Higher glycoprotein recovery when compared to other kits
Glycoprotein purification in less than an hour
Isolate glycoproteins from various sample types, eg. human serum and cell lysate
Lectin does not leach from resin when processing sample
Dialysis or protein precipitation of recovered glycoproteins is not required prior to protein assay

: Sufficient for isolating glycoproteins with strong affinity for ConA (or WGA) from 10 samples of up to 640 µl (1 to 1,5 mg total protein) each. Kit contains ConA Lectin Resin (or WGA Lectin Resin), 1,1 ml; Binding/Wash Buffer (5X), 6,5 ml; Elution Buffer, 5 ml; Spin columns (10); and collection tubes (20).