Nerve growth factor Receptor (NGFR/P75ECD) Rapid™ ELISA kit: Mouse

The Biosensis NGFR/p75ECD Rapid™ enzyme-linked immunosorbent assay (ELISA) kit is a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of mouse p75 neurotrophin receptor in less than 4 hours in cell culture supernatants and urine only if used as directed.

This NGFR/p75^ECD ELISA kit employs a recombinant mouse p75^ECD-Fc chimera as standard. While there is a current lack of a true mouse p75^ECD standard, this ELISA kit allows quantification of mouse p75^ECD as p75^ECD–Fc mouse equivalents. Please note that the antibodies used in this ELISA cross-react with human NGFR/p75^ECD.

The purpose of this kit is the in vitro quantitative determination of mouse p75^ECD in samples such as cell culture supernatants and urine. This kit has not been tested for other applications.

This kit consists of a pre-coated mouse monoclonal anti-p75^ECD capture antibody, a goat anti-p75^ECD detection antibody and a horseradish peroxidase (HRP)-conjugated anti-goat antibody. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of p75^ECD present in samples and protein standards.

Please refer to the kit protocol for specific use instructions for each substrate application.

: Each kit contains 1 or 2 × 96 pre-coated strip plates, protein standards, detection reagents, wash and sample buffers and detailed instructions.

: For research use only. Not for diagnostic or clinical purposes.

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Assay Type:
Sandwich
Primary antibody reactivity:
Mouse
Cross reactivity:
The antibodies used in this ELISA kit does not cross-react with human p75^ECD protein
Detection range:
62,5 – 4000 pg/ml
Sensitivity:
Typically detects down to 20 to 40 pg/ml of mouse p75^ECD (defined as blank OD plus 3x the standard deviation of the blank OD, n=10)