Glial cell line-derived neurotrophic factor (GDNF) Rapid™ ELISA kit: human

The Biosensis GDNF Rapid™ enzyme-linked immunosorbent assay (ELISA) kit is a sandwich ELISA that allows the quantification of GDNF in less than 4 hours in cell culture supernatants, cell lysates, and guinea pig serum only if used as directed.

This ELISA kit consists of a pre-coated anti-GDNF capture antibody, a biotinylated anti-GDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’- tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of GDNF present in samples and protein standards. A GDNF positive control (QC sample) is provided to assure consistent assay performance.

This GDNF ELISA kit employs a recombinant human GDNF standard obtained from the National Institute for Biological Standards and Control (NIBSC) and is therefore designed to accurately measure the human form of GDNF. Note that the antibodies used in this kit cross-react with guinea pig, rat and mouse GDNF

Guinea pig GDNF has been quantified in serum using the Human GDNF RapidTM ELISA kit, since guinea pig GDNF protein shares closest amino acid sequence homology with human GDNF. Protein levels can be expressed as 'human GDNF equivalents'.

This kit has not been tested for other applications.

Please refer to the kit protocol for specific use instructions for each substrate application.

: Each kit contains 1 or 2×96 pre-coated strip plates, protein standards, detection reagents, wash and sample buffers and detailed protocols.

: For research use only. Not for diagnostic or clinical purposes.

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Assay Type:
Sandwich
Primary antibody reactivity:
Human
Target protein:
GDNF
Cross reactivity:
No cross-reactivity was observed for the following proteins tested at 25 ng/mL: brain-derived neurotrophic factor (rhBDNF), nerve growth factor (rhNGF), neurotrophin-3 (rhNT-3), rhNT-4/5, vascular endothelial growth factor (rhVEGF), recombinant human Neurturin, Artemin and Persephin.
Specificity:
Human. The antibodies used in this kit detect mouse and rat GDNF.
Detection range:
7,8 – 500 pg/ml
Sensitivity:
Typical limit of detection (LOD) for rhGDNF is <8 pg/ml determined as blank value plus 3× standard deviation of blank OD (n=10).