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Specifications
- Antibody type:Secondary
- Antigen name:Immunoglobulin G
- Antigen symbol:IgG F(c)
- Clonality:Polyclonal
- Conjugation:TRITC (Tetramethylrhodamine Isothiocyanate)
- ELISA:Yes
- Flow cytometry:Yes
- Host:Goat
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- ImmunoPrecipitation:Yes
- Isotype:IgG
- Reactivity:Rabbit
- Western blot:Yes
- Epitope:F(c)
- Cross adsorption:Yes
- Format:Purified IgG
- Form:Lyophilised
- Antibody fragment:F(c)
- Storage buffer:10 mM sodium phosphate, 0,15 M sodium chloride, pH 7,2, 1 % (w/v) BSA, protease/IgG free
- Storage temperature:2...8 °C
- Concentration:1,0 mg/ml (E 1% at 280 nm = 13,0)
- Shipping temperature:2...8 °C
- Immunogen:Rabbit IgG Fc
- Tested applications:Immunomicroscopy
- Purification:Affinity purified using solid phaseRabbit IgG
- Size:1,0 mg
- Pk:1 mg
Specifications
About this item
TRITC (Tetramethylrhodamine isothiocyanate) is a popular rhodamine derivative pink in colour but emits a red-orange colour upon Emission at 580 nm. TRITC is frequently paired with FITC in double labeling experiments and conjugated antibodies are often used in immunofluorescence microscopy, flow cytometry, FLISA (fluorescent ELISA) and high throughput screening assays. These conjugates are best excited with the 532 nm spectral line of the He-Ne laser and are also recommended as a second colour detection reagent in in situ hybridisation applications. (Excitation/Emission = 555 nm/580 nm Emission Colour = Orange to Red (Similar Dyes: Alexa Fluor 555, Cy3, DyLight 550)
- Origin: US
ImmunoReagents' antibodies are suitable for most immunoassay formats and purified, cross adsorbed and conjugated based on the final product specfications.
Products will be accompanied with a standard product CoA to include application, purity and cross reactivity profiles.
Type: Secondary
Antigen: IgG F(c)
Clonality: Polyclonal
Clone:
Conjugation: TRITC (Tetramethylrhodamine Isothiocyanate)
Epitope:
Host: Goat
Isotype: IgG
Reactivity: Rabbit