NF[kappa]B transcription factor assay kits
ROCKKAA064
:
- Pk:1 KIT
- Description:NF[kappa]B P50 assay kit
These assays are non-radioactive methods for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A specific dsDNA sequence containing the NF[kappa]B response element is immobilized on the bottom of the wells in a 96-well plate. The transcription factor is detected by the addition of a specific primary antibody. A colorimetric readout at 450 nm is obtained through a horseradish peroxidase-conjugated secondary antibody.
: Includes transcription factor A, NF[kappa]B p50 or NF[kappa]B p65 positive control and primary antibody, HRP-conjugated goat anti-rabbit secondary antibody, specific competitor dsDNA, antibody binding buffer (10X), binding assay (4X), wash buffer concentrate (400X), Tween®-20 polysorbate, developing solution, stop solution, and 96-well plate and plate cover.