You searched for: Nucleic Acid Reagents

Supplier: Cytiva

The illustra™ triplePrep Kit is designed for the rapid, simultaneous extraction and isolation of high yield genomic DNA (gDNA), total RNA, and total denatured proteins from undivided animal tissues and mammalian cells. The streamlined workflow reduces the overall number of steps, enabling the preparation of all three analytes in less than one hour.

Supplier: Corning

AxyPrep™ Mag Plasmid Purification Kit utilises a magnetic bead technology for high throughput purification of plasmid DNA from E.coli cells. The AxyPrep™ Mag kit can also be used with fosmid and BAC vector-based constructs ranging from 5 to 150 Kb. The system consists of steps of pelleting cells, resuspension, lysis, neutralisation, DNA binding, ethanol washing and plasmid elution. The protocol is automation-compatible.

Supplier: Cytiva

illustra™ tissue and cells genomicPrep Mini Spin Kit is designed for the rapid extraction and purification of high quality genomic DNA from a variety of animal tissues and mammalian cell cultures. The kit can be used for several downstream applications including restriction enzyme analysis, ligation, cloning, DNA sequencing and PCR.

Supplier: Cytiva

Sera-Mag Select PCR clean-up, size selection reagent combines the exceptional binding characteristics of Sera-Mag carboxyl speedbeads with an optimised binding solution in a ready to use formulation. The DNA size range isolated from the process can be tailored to suit the user’s end requirements by adjusting the amount of reagent that is mixed with a fixed volume of sample.

Supplier: Merck Millipore (Novagen)

The One Step RT-PCR Master Mix Kit allows rapid, sensitive analysis of gene expression from tissues and cells. It can replace methods for detecting and quantifying gene expression such as Northern blots, in situ hybridisation, dot blots, S nuclease assays and conventional two step RT-PCR. The kit utilises recombinant Thermus thermophilus (rTth) DNA Polymerase, which acts as both a thermostable RNA dependent DNA polymerase and a DNA dependent DNA polymerase. The rTth DNA Polymerase is provided in a 2X master mix with an antibody for antibody-mediated hot start, optimised buffer, and ultrapure deoxynucleotides. Antibody-mediated Hot Start enhances specificity of both reverse transcription and PCR. The kit enables cDNA synthesis from input RNA followed by PCR amplification of the cDNA in a single reaction, with no additional hands-on requirement for buffer changes or adding reagents. Typically, detection of a specific transcript requires only 2 hours.

Supplier: Cytiva

NICK™ columns were originally designed for purification of nick-translated DNA fragments but are also suitable for separation of any labelled probe from unincorporated labelled nucleotides.

Supplier: G-Biosciences

The majority of genomic DNA extraction methods involve numerous physical manipulations, including mixing, pipetting, shaking, binding to resin, elution, which results in sheared DNA that may not be suitable for further analysis.

Supplier: MP Biomedicals

The MagBeads FastDNA Kit for Feces allows thorough sample lysis and quick isolation of DNA from fecal samples. It is using magnetic beads with high DNA binding capacity for extraction of high yields of pure DNA. This is done manually using a magnetic rack or adapted to automated instruments for high throughput processing.

Supplier: MP Biomedicals

MagBeads Fast Circulating DNA Kit is intended for purification of high-quality circulating DNA (cfDNA) from cell-free body fluids (such as plasma, serum). The purified DNA is suitable for direct use in downstream applications such as PCR, real-time PCR, Biochip analysis and NGS.

Supplier: MP Biomedicals

Host DNA contamination impedes the molecular analyses of microbiomes in host samples, such as bodily fluids and swabs. The SPINeasy Host Depletion Microbial DNA kit provides an easy-to-use workflow to isolate microbial DNA from samples containing high amounts of host DNA.

Supplier: Cytiva

illustra™ AutoSeq™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high quality sequencing results since residual dye-terminators can obscure data. illustra™ AutoSeq™ G-50 consists of MicroSpin™ columns containing Sephadex™ G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.

Supplier: MP Biomedicals

MagBeads FastDNA/RNA Kit for Virus is intended for extracting total viral nucleic acid from cell-free/low-cell content biological samples such as body fluids, serum, plasma, soaking solution, tissue homogenate, and culture supernatant.

Supplier: Cytiva

Sephadex™ G-100 DNA Grade is ideal for use in preparing spin columns for DNA purification.

Supplier: Cytiva

Nucleon™ PhytoPure™ enables rapid chloroform extraction of high-quality, high molecular weight genomic DNA from plant and fungal samples with efficient removal of protein as well as polysaccharides.

Supplier: MP Biomedicals

The FastDNA™ SPIN Kit for Soil is designed to efficiently isolate bacterial, fungi, plant and animal genomic DNA from soil and environmental samples.

Supplier: Cytiva

illustra™ MicroSpin™ S-300 HR columns designed for rapid purification of PCR products (>100 bp) from unincorporated primers (<20-mers) and nucleotides using spin-column chromatography.

Supplier: Cytiva

RNAspin Mini RNA Isolation Kit is a complete RNA purification kit designed for rapid extraction of high-quality total RNA from a wide range of sample types.

Supplier: Cytiva

Sera-Xtracta Cell-Free DNA Kit for the efficient extraction and purification of cell-free DNA (cfDNA) from plasma.

Supplier: MP Biomedicals

MagBeads FastDNA® Kit for Blood is intended for purification of total DNA for reliable PCR and Southern blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from whole blood, plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.

Supplier: Corning

Axygen® AxyPrep™ Mag Plant gDNA Kit is a magnetic bead-based purification system that allows rapid and reliable isolation of high quality gDNA from a wide variety of plant samples. Up to 96 samples (50 mg fresh tissue per sample or 15 mg of dried tissue per sample) can be processed in less than one hour. The Axygen® AxyPrep™ Mag Plant gDNA Kit eliminates polysaccharides, phenolic compounds and enzyme inhibitors with no organic extractions. This kit is adaptable for use on major liquid handling workstations for high throughput processing.

Supplier: Cytiva

For purification of DNA (10 bases in length) from small molecules by gel filtration.

Supplier: Cytiva

For rapid purification of labelled DNA (≥20 bases) from unincorporated labelled nucleotides in a volume of 25 to 50 μl using spin column chromatography.

Supplier: MP Biomedicals

MagBeads FastRNA Kit is intended for simple and rapid extraction of total RNA from tissue and culture cells samples.

Supplier: MP Biomedicals

Quick and convenient purification of total RNA from gram-positive and gram-negative bacteria using spin column technology.

Supplier: Cytiva

Sephadex G-50 grade F is a size exclusion chromatography resin designed for desalting and buffer exchange, and for purification of DNA and oligonucleotides greater than 20 bases in length using spin columns.

Supplier: BIOPREMIER

The blood DNA extraction Kit is designed for the rapid purification of pure genomic DNA from whole blood, serum, plasma, body fluids and dried blood spots.

Supplier: Corning

The AxyPrep™ Mag Plasmid purification kit utilises a magnetic bead technology for high-throughput purification of plasmid DNA from E.coli cells.

Supplier: Cytiva

For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin column chromatography.

Supplier: MP Biomedicals

MagBeads FastDNA® Kit is intended for rapid extraction of DNA from tissue, cells, blood, saliva, swabs, blood spots, semen, and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, detection of viral DNA and so on.

Supplier: MP Biomedicals

SPINeasy Plasmid Miniprep kit is a silica-membrane spin-column kit that enables up to 20 µg of plasmid DNA to be purified from 1 to 5 ml of transformed bacterial culture.