You searched for: Nucleic Acid Reagents

Supplier: Merck Millipore (Novagen)

Pellet Paint® Co-Precipitant is a visible dye-labelled carrier formulated specifically for use in alcohol precipitation of nucleic acids. The 2-minute precipitation does not require low-temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even the most dilute solutions (2 ng/ml) and the pellet is easily located by its vivid pink colour. The pellet can be easily followed during washing steps and this prevents losses during handling and ensures thorough pellet re-suspension.

Supplier: Merck Millipore (Novagen)

The SpinPrep™ PCR Clean Up Kit is designed for the rapid purification of DNA amplified by PCR. The 10-minute procedure involves addition of a binding buffer followed by adsorption of the DNA to a silica membrane in a spin column format. Following a wash step, the DNA is eluted in low salt buffer. This kit removes DNA polymerises, dNTPs, salts, and >99% of primers so that they do not interfere with downstream applications such as cloning, sequencing, or labelling. PCR products from 100 to >12000 bp can be cleaned up, with recoveries of amplified DNA in the range of 60 to 90% under standard conditions.

Supplier: G-Biosciences

G-Biosciences offers a wide selection of genomic isolation kits that purify high quality genomic DNA from a variety of sources and for a wide array of applications. G-Biosciences XIT™ DNA kits produce protein-free, high quality DNA through the principle of cell lysis, protein digestion and precipitation and genomic DNA purification. No chloroform or phenol extraction is required. High quality DNA can be isolated from sample types including: animal tissues, cells, whole blood, bacteria, buccal cells, plant tissues, mouse tail, yeast and FFPE tissue. G-Biosciences XIT™ DNA kit procedures remove contaminants and enzyme inhibitors allowing the purified DNA to be ready for immediate use for all downstream analyses. The purified DNA from G-Biosciences XIT™ DNA kits have a A₂₆₀/A₂₈₀ ratio between 1,7 and 1,9 (with the exception of the buccal cells kit with a ratio between 1,8 and 2,0) and are up to 200 kb in size.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix provides all cDNA synthesis reaction components in a convenient one-tube master mix. It is optimised for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications.

Supplier: Quantabio

No compromises. Unparalleled performance meets sustainability.

Supplier: G-Biosciences

Fast Yeast Transformation™ is a rapid single step yeast transformation kit that takes less than 10 minutes to prepare competent yeast cells. The competent yeast cells can be used immediately or frozen for later use. This method is suitable for both circular and linear plasmid transformations.

Supplier: BTX

The BTXpress™ is a single buffer solution, developed to quickly and efficiently deliver genes into mammalian cells that were previously considered hard to transfect by chemical and other non viral methods. Transfection using this high performance electroporation solution is equally effective in delivering DNA as well as siRNA into mammalian cells.

Supplier: BTX

Flatpack chambers are primarily used for prokaryotic applications (bacterial or yeast transformations), however they are also often used for high efficiency stem cell transfection.

Supplier: AGILENT

High-quality polymerases for difficult to amplify DNA targets.

Supplier: Quantabio

Superior speed and inhibitor tolerance.

Supplier: AGILENT

Brilliant II SYBR® QPCR and QRT-PCR master mix kits with high/low Rox offer superior sensitivity for improved quantification and reproducibility.

Supplier: AGILENT

Increases specificity and yield of primary PCR amplification products.

Supplier: AGILENT

This DNA polymerase is ideal for a variety of techniques requiring high-fidelity DNA synthesis by the polymerase chain reaction (PCR).

Supplier: Thermo Fisher Scientific

For transformation of competent cells.

Supplier: AGILENT

Brilliant III Ultra-Fast SYBR® QPCR and QRT-PCR Master Mixes deliver superior specificity by utilizing a novel faster-activating hot start method to minimize the formation of primer-dimers and other off-target reactions.

Supplier: Quantabio

Qscript lyo 1-step is a lyophilised single-reaction reagent optimised for highly sensitive and reproducible one-step RT-qPCR using hydrolysis probes.

Supplier: Quantabio

Superior sensitivity for viral RNA detection.

Supplier: AGILENT

Brilliant II QPCR and QRT-PCR reagents were developed to maintain the high quality of original Brilliant product line, but with a significant improvement in sensitivity of detection, ensuring reproducible quantification even at low target concentrations.

Supplier: AGILENT

Herculase II Fusion DNA Polymerases produce superior yields with extension times as short as 15 s/kb, even with genomic DNA targets up to 23 kb.

Supplier: Quantabio

PerfeCTa® Multiplex qPCR SuperMix, low ROX is a 2X concentrated, ready to use reaction cocktail that contains all components, except primers, probe(s), and template for real time qPCR on Applied Biosystems 7500 or Stratagene MX series of real time PCR systems.

Supplier: Thermo Fisher Scientific

The RevertAid™ First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. The kit uses RevertAid™ Reverse Transcriptase which has lower RNase H activity compared to AMV reverse transcriptase. The recombinant RiboLock™ RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55 °C.

Supplier: Thermo Fisher Scientific

The Maxima™ First Strand cDNA Synthesis Kit is a convenient system optimised for use as a first step in two step RT-qPCR. The kit includes the Maxima™ Reverse Transcriptase, which is an advanced enzyme derived by in vitro evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR.

Supplier: VWR Chemicals

Hot Start PCR-To-Gel Taq Master Mix, 2X is a ready to use reaction cocktail that contains all required components, except primers and template DNA, for routine PCR amplification of DNA fragments up to 4 kb.

Supplier: AGILENT

MVP RNA is application-ready RNA available in small economical pack sizes.

Supplier: AGILENT

Detect as low as 300 fg of Porcine DNA in food and pharmaceutical samples, including gel caps.

Supplier: AGILENT

Improves reliability and yield of conventional Taq-based PCR amplifications.

Supplier: AGILENT

Cloned Pfu DNA Polymerase for high fidelity and difficult, GC-Rich PCR.

Supplier: Biotium

A qPCR master mix containing Cheetah™ Taq hotstart DNA polymerase, suitable for probe-based real-time PCR applications.

Supplier: BTX

BTXpress Cytoporation® Media T/T4 is an advanced electroporation buffer designed for use with the BTX AgilePulse MAX™ large volume electroporation system for in vitro delivery of DNA, RNA, oligonucleotides, and siRNA. The low conductivity buffer is specially formulated to minimise heating of solution during large volume electroporation for maximum transfection efficiency and high cell viability. BTXpress Cytoporation® Media T and T4 are sterile filtered from the highest quality non animal, medical grade reagents. Buffer can be directly diluted in complete growth media for post electroporation cell culture.

Supplier: AGILENT

Easily integrates into PCR protocols optimized with Taq DNA polymerase, with little or no modification.