You searched for: Nucleic Acid Reagents

Supplier: G-Biosciences

GET™ AGAROSE DNA method involves release of DNA fragments from gel pieces followed by capture of nucleic acids on the GET™ spin columns, washing, and elution of the clean nucleic acid fragments in a suitable buffer.

Supplier: VWR Chemicals

This fast extract genotyping PCR kit consists of a fast extraction DNA solution and VWR® Red Taq DNA polymerase 2X master mix. It is ideal for genotyping of DNA extracted from mammalian tissues, plant material, saliva or bacteria, providing PCR-ready DNA in 8 minutes and genotyping results in less than 1,5 hours.

Supplier: VWR Chemicals

Highly concentrated Taq DNA polymerase glycerol free 50 U/µl is ideal for industrial use and manufactures of diagnostic PCR kits.

Supplier: VWR Chemicals

Fast HiFi DNA Polymerase 2 U/µl is a proof reading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning/subcloning, NGS applications and mutagenesis.

Supplier: VWR Chemicals

VWR® One-Step RT-qPCR Kit is designed to facilitate rapid, sensitive, and precise detection and quantification of various RNA sequences via dye-based qPCR. It features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

Supplier: VWR Chemicals

Fast HiFi DNA Polymerase 2 U/µl is a proofreading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning / sub cloning, NGS applications and mutagenesis.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix provides all cDNA synthesis reaction components in a convenient one-tube master mix. It is optimised for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix with dsDNase provides a simple workflow that combines genomic DNA elimination and cDNA synthesis in a one-tube procedure. The cDNA reaction components are pre-mixed into a complete master mix that is convenient to use, reduces pipetting steps, and is optimised for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications. Maxima™ H Minus cDNA Synthesis Master Mix is optimised for reproducible cDNA synthesis at elevated temperatures (50 to 65°C) within 30 minutes.

Supplier: Thermo Fisher Scientific

Maxima™ H Minus First Strand cDNA Synthesis Kit is a complete system for highly efficient synthesis of first strand cDNA. The kit uses the Maxima™ H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived by in vitro evolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima™ H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65 °C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.

Supplier: Thermo Fisher Scientific

RevertAid™ Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids, which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Supplier: Thermo Fisher Scientific

Random hexamer primer is a mixture of single-stranded random hexanucleotides with 5'- and 3'-hydroxyl ends. The primer is supplied as a ready to use, 20X concentrated aqueous solution. Random hexamer primer has been functionally tested in first strand cDNA synthesis.

Supplier: Thermo Fisher Scientific

5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression. The EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyse DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5’-CCGG-3’ tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected. The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.

Supplier: Thermo Fisher Scientific

Oligo(dT)₁₈ primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends. The primer is supplied as a ready to use, 20X concentrated aqueous solution. Oligo(dT)₁₈ primer has been functionally tested in first strand cDNA synthesis.

Supplier: Thermo Fisher Scientific

The First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates. The kit uses M-MuLV Reverse Transcriptase, which has lower RNase H activity compared to AMW reverse transcriptase. The enzyme maintains activity at 37 °C and is suitable for synthesis of cDNA up to 9 kb. The recombinant Thermo Scientific RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55 °C.

Supplier: BTX

Flatpack chambers are primarily used for prokaryotic applications (bacterial or yeast transformations), however they are also often used for high efficiency stem cell transfection.

Supplier: G-Biosciences

Fast Yeast Transformation™ is a rapid single step yeast transformation kit that takes less than 10 minutes to prepare competent yeast cells. The competent yeast cells can be used immediately or frozen for later use. This method is suitable for both circular and linear plasmid transformations.

Supplier: BTX

The BTXpress™ is a single buffer solution, developed to quickly and efficiently deliver genes into mammalian cells that were previously considered hard to transfect by chemical and other non viral methods. Transfection using this high performance electroporation solution is equally effective in delivering DNA as well as siRNA into mammalian cells.

Supplier: Thermo Fisher Scientific

For transformation of competent cells.

Supplier: Thermo Fisher Scientific

RevertAid™ H Minus Reverse Transcriptase (RT) is a genetically modified M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to point mutation in the RNase H domain. RevertAid™ H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full-length cDNA from long templates are obtained. RevertAid™ H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 55 °C), which makes the enzyme an ideal tool for reverse transcription of RNAs having a high degree of secondary structure.

Supplier: Thermo Fisher Scientific

Maxima™ Reverse Transcriptase is an advanced enzyme derived by in vitro evolution of M-MuLV RT for RT-qPCR application. The enzyme features high thermostability, robustness and increased synthesis rates in RT-qPCR. The Maxima™ Reverse Transcriptase is capable of cDNA synthesis at elevated temperatures (50 to 60 °C) and completion of the reverse transcription reaction in 15 to 30 minutes. The use of this enzyme in combination with Maxima™ qPCR Master Mixes ensures high specificity, sensitivity and wide linear range of two step RT-qPCR. Applications include first strand cDNA synthesis, RT-PCR, and RT-qPCR.

Supplier: Thermo Fisher Scientific

RevertAid™ H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid™ H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

Supplier: VWR Chemicals

VWR® qPCR Master Mix features a distinctive universal passive reference dye that is compatible with ROX-dependent and ROX-independent qPCR instruments.

Supplier: AGILENT

Polyadenylation of miRNA from total RNA and synthesis of first-strand cDNA from tailed miRNAs.

Supplier: G-Biosciences

OmniPrep™ for Plant Kit isolates high quality genomic DNA from plant samples, including fresh and frozen plant tissues. If used according to the protocols this kit purifies DNA from 20gm plant tissue.

Supplier: G-Biosciences

OmniPrep™ for Blood Kit isolates high quality genomic DNA from blood samples, including whole blood, buffy coats, packed cells and bone marrow.

Supplier: G-Biosciences

OmniPrep™ for Yeast Kit isolates high quality genomic DNA from yeast. When used according to the recommended protocols, this kit purifies DNA from 300 ml yeast culture.

Supplier: AGILENT

The brilliant multiplex QPCR master mix allows you to amplify up to four targets in a single real-time PCR Reaction.

Supplier: AGILENT

The Brilliant HRM Ultra-Fast Loci Master Mix for High Resolution Melt (HRM) analysis.

Supplier: Merck Millipore (Novagen)

Pellet Paint® Co-Precipitant is a visible dye-labelled carrier formulated specifically for use in alcohol precipitation of nucleic acids. The 2-minute precipitation does not require low-temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even the most dilute solutions (2 ng/ml) and the pellet is easily located by its vivid pink colour. The pellet can be easily followed during washing steps and this prevents losses during handling and ensures thorough pellet re-suspension.

Supplier: Thermo Fisher Scientific

The RevertAid™ First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. The kit uses RevertAid™ Reverse Transcriptase which has lower RNase H activity compared to AMV reverse transcriptase. The recombinant RiboLock™ RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55 °C.