You searched for: Nucleic Acid Reagents
Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.
dTTP *100 mM PCR Grade*
Supplier: AAT Bioquest
2'-Deoxythymidine-5'-Triphosphate (dTTP) solution is used in DNA synthesis reactions such as PCR, DNA sequencing and molecular cloning techniques.
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dATP *100 mM PCR Grade*
Supplier: AAT Bioquest
2′-Deoxyadenosine 5′-triphosphate sodium salt (dATP) solution is used in DNA synthesis reactions such as PCR, DNA sequencing and molecular cloning techniques.
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3'-O-Azidomethyl-dCTP
Supplier: AAT Bioquest
3'-O-Azidomethyl-dCTP is one of the four essential dNTPs (A, C, G, T) that can be used for the DNA sequencing by synthesis (SBS).
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Random mutagenesis kits geneMorph II
Supplier: AGILENT
The GeneMorph II random mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis.
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T7 RNA polymerase
Supplier: Thermo Fisher Scientific
T7 RNA polymerase is ideal for synthesis of unlabeled and labeled RNA. It catalyses the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter. It is also used in the study of RNA secondary structure and RNA-protein interactions, RNA splicing.
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dGTP *100 mM PCR Grade*
Supplier: AAT Bioquest
2'-Deoxyguanosine-5'-Triphosphate (dGTP) solution is used in DNA synthesis reactions such as PCR, DNA sequencing and molecular cloning techniques.
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iFluor® 560 dUTP *1 mM in TE Buffer (pH 7,5)*
Supplier: AAT Bioquest
iFluor® 560-dUTP is a iFluor 560-modified nucleotide that can be used in various molecular biology applications, such as labelling and detection of DNA molecules.
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Fluorescein-12-dGTP *1 mM in Tris Buffer (pH 7,5)*
Supplier: AAT Bioquest
Fluorescein-12-dGTP might be incorporated into a DNA sequence as its natural counterpart dGTP to incorporate a fluorescein tag in a DNA molecule.
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ReadiUse™ ddNTP Terminator Mix *10 mM*
Supplier: AAT Bioquest
ReadiUse™ ddNTP Terminator Mix consists of 4 equimolar 2',3'-ddNTPs, including ddATP, ddGTP, ddTTP and ddCTP.
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Packaging extracts, Gigapack III
Supplier: AGILENT
For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency and representation of libraries constructed from highly methylated DNA. Each packaging extract is restriction minus to optimize packaging efficiency and library representation.
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QuikChange HT protein engineering system
Supplier: AGILENT
Protein directed evolution in just one step.
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ddATP [2',3'-Dideoxyadenosine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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2-Aminoethoxypropargyl ddTTP (1 µmole)
Supplier: AAT Bioquest
Sanger method is one of the most reliable and earliest DNA sequencing methods.
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2-Aminoethoxypropargyl ddATP (1 µmole)
Supplier: AAT Bioquest
Sanger method is one of the most reliable and earliest DNA sequencing methods.
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MagaDye™ 561 ddATP
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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DNA ligation kit
Supplier: AGILENT
The DNA Ligation Kit includes T4 DNA ligase for highly efficient ligations.
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PCR cloning kit, CloneJET
Supplier: Thermo Fisher Scientific
The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host E. coli cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.
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Mutagenesis kits QuikChange
Supplier: AGILENT
The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. In addition, the XL version of the kit is specially designed for efficient mutagenesis of large (4 to 14 kb) or otherwise difficult-to mutagenise plasmid templates. The XL kit features components specifically designed for more efficient DNA replication and bacterial transformation.
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Pfu DNA ligase
Supplier: AGILENT
Pfu DNA ligase is a recombinant enzyme derived from Pyrococcus furiosus hyperthermophilic marine archaebacterium that catalyses linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45 to 80 °C, with a temperature optimum near 70 °C for nick-sealing reactions.
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PCR cloning kits, StrataClone
Supplier: AGILENT
The StrataClone PCR cloning kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase.
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2-Aminoethoxypropargyl ddGTP (1 µmole)
Supplier: AAT Bioquest
Sanger method is one of the most reliable and earliest DNA sequencing methods.
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MagaDye™ 588 ddTTP
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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ddCTP [2',3'-Dideoxycytidine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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ddGTP [2',3'-Dideoxyguanosine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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ddTTP [2',3'-Dideoxythymidine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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MagaDye™ 535 ddGTP
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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MagaDye™ 613 ddCTP
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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2-Aminoethoxypropargyl ddCTP (1 µmole)
Supplier: AAT Bioquest
Sanger method is one of the most reliable and earliest DNA sequencing methods.
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Genomic DNA isolation From mouse tail, OmniPrep™
Supplier: G-Biosciences
OmniPrep™ for Mouse Tail Kit isolates high quality genomic DNA from mouse tail samples. If used according to the protocols this kit purifies DNA from 100 to 200 cm mouse tail.
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Plant Genomic DNA purification Mini kit, GeneJET™
Supplier: Thermo Fisher Scientific
GeneJET™ Plant Genomic DNA Purification Mini Kit is designed for rapid and efficient purification of high quality genomic DNA from a wide variety of plant species and tissue types. It utilises silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 30 minutes following cell lysis and yields purified DNA greater than 30 kb in size. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, and the age of the tissue sample. The typical yield from a standard tissue source (for example, young wheat leaves) is 30 to 32 μg from 100 mg of tissue. Isolated DNA can be used directly in PCR, qPCR, Southern blotting, and enzymatic reactions.