You searched for: Chromatography Columns

Supplier: VARIAN

The CP-Chirasil-Dex CB phase consists of cyclodextrin directly bonded to dimethylpolysiloxane. This bond prevents the cyclodextrin from migrating to different locations in the surface film, delivering homogeneous enantioselectivity throughout the phase.

Supplier: VARIAN

The CP-Si™l line consists of a range of stationary phases which are based on a polysiloxane backbone. Functional groups are added to the polymer to introduce selectivity. Each polymer used for the CP-Sil line is manufactured in-house and is optimised to obtain highest degree of reproducibility.

Supplier: VARIAN

Bond Elut™ PSA is an alkylated amine sorbent that contains two different amino functionalities, one secondary and one primary. This gives a slightly higher pKa and ionic capacity compared to Bond Elut™ NH₂. The PSA functional group is a very effective bidentate ligand and is therefore an excellent sorbent for chelation applications. PSA has a significantly higher carbon load than most amino functional sorbents and thus is a better choice for polar compounds, which retain too strongly on Bond Elut™ NH₂.

Supplier: VARIAN

The Bond Elut™ Certify extraction cartridge utilises a packed bed consisting of a special, non-polar C8 sorbent and a strong cation exchanger (SCX). Certify is most commonly used to extract basic (cationic) drugs from urine and blood, but it is also very effective for extraction of a wide range of compounds from a diverse range of aqueous matrices.

Supplier: VARIAN

Bond Elut™ Diol (2OH) resembles unbonded silica in its tendency for strong hydrogen bonding with analytes. 2OH can also be employed in the non-polar mode because the hydrocarbon spacer on its functional group provides enough non-polar character for retention of hydrophobic analytes. Bond Elut™ Diol is a listed SPE device for the DIN 14333-1 method on benzimidazole fungicides.

Supplier: VARIAN

Bond Elut CN-U is ideally suited for the extraction of polar compounds from a nonpolar matrix, such as hexane or oils. It is suitable for very polar analytes that may be irreversibly retained on SI or Diol (2OH) SPE phases.

Supplier: VARIAN

Bond Elut™ Jr cartridges have top and bottom Luer fittings allowing easy sample processing when used in conjunction with standard SPE cartridges. Bond Elut™ LRC cartridges have a large reservoir above the sorbent bed and are suitable for use on any standard SPE vacuum manifold.

Supplier: VARIAN

The VF-5ht improves the analysis of high boiling compounds by exhibiting low bleed, even at high temperatures. Based on ultra-low bleed FactorFour technology, VF-5ht provides unmatched selectivity, sensitivity, and accuracy for the analysis of high-molecular weight compounds.

Supplier: VARIAN

Bond Elut™ LMS polymeric sorbent lets you elute without having to add amine modifiers, buffers, or acids. The elimination of secondary interactions means that elution of analytes can be achieved with pure organic solvents or HPLC mobile phase-compatible solvent mixtures of low ionic strength. These characteristics are crucial to allow compatibility with LC/MS or other delicate analytical techniques.

Supplier: VARIAN

A medium polarity sorbent with many uses, Bond Elut™ CN-E is ideal for applications in which extremely non-polar compounds would be irreversibly retained on high carbon load sorbents such as C₈ and C₁₈. This endcapped version of the cyano sorbent is best utilised when extracting analytes from an aqueous matrix.

Supplier: VARIAN

Bond Elut™ Plexa PCX uses a polymeric cation exchange resin that combines the outstanding properties of Bond Elut™ Plexa - superior flow characteristics and improved analytical performance - with strong cation exchange functionalities. This mixed-mode SPE sorbent removes neutral and acidic interferences from the matrix, concentrates basic analytes and therefore improves sensitivity in the determination of basic compounds.

Supplier: VARIAN

Bond Elut™ DEA bears some resemblance to Bond Elut™ NH₂ in its properties but with a slightly lower capacity as an anion exchange sorbent. DEA has a moderately non-polar character due to the alkyl side chains on the amino functionality. These groups still afford a medium level of polarity, higher than C₈ but less polar than C₂ or CN-E.

Supplier: VARIAN

Bond Elut™ C18 is the most hydrophobic, bonded silica sorbent in the Bond Elut™ range. It is the most popular SPE sorbent because of its extremely retentive nature for non-polar compounds. C18 is generally regarded as having the broadest spectrum of retention among bonded silica sorbents, since it retains most organic analytes from aqueous matrices. When analysing small to intermediate molecules, Bond Elut™ C18 can be used for desalting aqueous matrices prior to ion exchange, as salts pass through the sorbent unretained.

Supplier: VARIAN

Bond Elut™ PPL is a styrene-divinylvenzene (SDVB) polymer that has been modified with a proprietary non-polar surface. PPL will retain even the most polar classes of analytes, including phenols. The large particle size allows ease of flow for viscous or particulate rich water samples, while the high surface area and strong hydrophobicity ensure reproducible extractions with high recoveries upon elution. Bond Elut™ PPL is suitable for EPA Method 528 ‘Determination of Phenols in Drinking Water by SPE and Capillary GC/MS’.

Supplier: VARIAN

Bond Elut™ NEXUS is an ultra-clean polymeric sorbent which has bi-modal porosity and a high surface area. NEXUS offers a nonpolar retention mechanism with no pre-conditioning required. The large particle size makes NEXUS ideal for extractions from highly viscous samples, such as horse urine.

Based on the same base polymer technology, Bond Elut™ NEXUS WCX is a weak cation exchange sorbent that offers extra selectivity for analytes, such as quaternary ammonium drugs and anabolic steroids.

Supplier: VARIAN

CP-Al₂O₃/Na₂SO₄ is an aluminum oxide PLOT column that offers high selectivity for separating ppm levels of C₁ to C₅ hydrocarbons in a main stream of C₁ to C₅ hydrocarbons. The Na₂SO₄ deactivation salt provides a polar Al₂O₃ surface, so that unsaturated compounds such as ethylene and acetylene are retained longer. These columns analyse more compounds in a single run than packed columns, while still delivering higher resolution and faster analysis times. When compared to liquid stationary phases, CP-Al₂O₃ provides increased selectivity and allows all C₁ to C₅ hydrocarbon isomers to be separated. CP-Al₂O₃ operates without the need for sub-ambient cooling.

Supplier: VARIAN

Bond Elut™ SCX is a strong cation exchanger with a very low pKa. Although the pKa is similar to Bond Elut™ PRS, the presence of the benzene ring in the functional group increases the potential for non-polar interactions. This non-polar characteristic becomes particularly important when conducting ion-exchange from aqueous systems, where selectivity towards compounds exhibiting cationic and non-polar character is seen.

Supplier: VARIAN

Bond Elut™ PRS is a strong cation exchange sorbent that is also relatively high in polarity. With no appreciable degree of hydrophobicity, in non-polar solvents, PRS is capable of polar and hydrogen bonding interactions. Due to the very low pKa of PRS, a high ionic strength eluent must be used, and so the use of PRS is recommended for weaker cationic species such as pyridinium compounds.

Supplier: VARIAN

PBA is a unique sorbent consisting of a phenylboronic acid covalently linked to a silica gel surface. Covalent processes result in a higher retention to the sorbent surface than other mechanisms, resulting in superior clean-up. The boronate group has a high specificity for cis-diol containing compounds such as catechols, nucleic acids, low molecular weight proteins and carbohydrates.

Supplier: Agilent

Step 1: Extraction
Choose the extraction salt packet based on your method of analysis, AOAC or EN. The buffered extraction salts are amenable for more liable pesticides. Adding solvent and then salts to a comminuted fruit or vegetable sample (10 g or 15 g) enables you to extract the pesticides of interest into the organic layer. However, adding a food sample with a high percentage of water directly to the salts may create an exothermic reaction that can affect your analyte recoveries.

Supplier: VARIAN

The water-wettable, hydroxylated exterior allows excellent flow, even with biological fluids. A gradient of polarity on the polymer surface shunts small analytes to the more hydrophobic centre of the polymer bead, where they are retained prior to the washing and elution steps. Plexa provides these performance enhancements due to a unique polymeric architecture with a non-retentive, hydroxylated, amide-free surface and a non-polar PS/DVB core for retaining small molecules.

Supplier: VARIAN

Bond Elut™ Certify II was developed specifically for the rapid and effective extraction of acidic drugs and metabolites from urine and other biological matrices. Certify II is a mixed-mode cartridge packed with non-polar C8 and strong anion exchange (SAX) sorbent. It has been optimised for acidic drugs such as 11-nor-delta-9-tetrahydrocannibinol-carboxylic acid, salicylic acid, ibuprofen, acetaminophen and other compounds that possess both non-polar and anionic characteristics.

Supplier: VARIAN

Due to the methyl group and subsequent low carbon load, Bond Elut™ C1 is the least retentive of all alkyl group bonded phases for non-polar compounds. However, due to the extensive end capping of this sorbent to mask polar silanol activity, retention and elution of polar and multi-functional analytes can still be achieved. Typical applications include plasma, urine and aqueous samples.

Supplier: Agilent

Step 2: Dispersive SPE cleanup
Select the dispersive SPE kit suited to the type of food being analysed and the method you are following. In this step, an aliquot of the sample extract from step one (see QuEChERS extraction kits) is added to a 2 or 15 ml centrifuge tube containing a small amount of SPE sorbent and MgSO₄. The sorbent will pull out interfering matrix materials from the sample, while the MgSO₄ helps remove excess water and improve analyte partitioning.

Supplier: KNAUER

These columns with HPLC phase are perfectly suited to take on routine analyses as well as the most ambitious chromatography tasks.

Supplier: NEUBERT VOLUME GLASSWAERE

These chromatography columns feature a top sleeve with PTFE tap and are made from borosilicate glass 3.3.

Supplier: Uptima

These flash chromatography columns, irregular C18 silica serves a broad-ship of purification requirements for mid to non polar compounds.

Supplier: NEUBERT VOLUME GLASSWAERE

Glass, Empty columns: Yes, Ø int.: 200 mm, Length: 300 mm

Supplier: Restek

These are stationary phase columns made with high-purity and type-B silica that minimises activity and creates high-density bonding.

Supplier: Restek

Ultra PFP (pentafluorophenyl) Propyl preparative columns are ideal for the retention and selectivity of charged bases, electronegative compounds, and amine-containing compounds.