You searched for: Cellular Assays

Supplier: Enzo Life Sciences

For the quantitative determination of nitrite and nitrate levels in culture supernatants, plasma, serum, saliva, and urine from any species.

Supplier: Biotium

JC-1 dye is used for fluorescence-based measurement of mitochondrial membrane potential changes in cells, a key measure of cell viability.

Supplier: Enzo Life Sciences

GFP-Certified™ Apoptosis/Necrosis Detection Kit is a multiplex assay that distinguishes between healthy, early apoptotic, late apoptotic and necrotic cells, compatible with GFP and other green fluorescent probes.

Supplier: Biotium

Cell surface staining kits with fluorescent membrane dye plus enhancer for imaging live cell surface for several hours to days.

Supplier: Enzo Life Sciences

1. Ubiquitinylation of specific endogenous HeLa lysate proteins, followed by their immediate detection/analysis using antibodies to the protein/s of interest, indicating a particular protein is a substrate for the ubiquitin-proteasome pathway. 2. Ubiquitinylation of proteins of interest contained in exogenously added expression culture/cell extracts or tissue lysates/extracts, followed by their immediate detection/analysis or isolation/purification for use in subsequent experiments. 3. Modification of proteins using ubiquitin derivatives or mutants for improved detection/analysis or investigation of alternative (non-proteasomal) ubiquitin related pathways in subsequent experiments. For example, biotinylated-ubiquitin (Prod. No. BML-UW8705), for sensitive detection

Supplier: Biotium

NucView® 488 and CF™dye (594, 640R and Texas RED) conjugated annexin V provides two apoptosis events, caspase-3 activity and phosphatidylserine (PS) translocation.

Supplier: Cayman Chemical

96 well assay for measurement of SREBP-2.

Supplier: Enzo Life Sciences

GFP-Certified™ Nuclear-ID™ Red Cell Cycle Analysis Kit provides a convenient approach for studying the induction and inhibition of cell cycle progression by flow cytometry.

Supplier: Enzo Life Sciences

MITO-ID® Red Detection Kit (GFP-CERTIFIED®) is a photostable, non-toxic and selective mitochondrial dye that stains live, detergent permeabilised and aldehyde fixed cells. This kit is specifically designed for use with GFP expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFP's, CFP's, YFP's).

Supplier: Cayman Chemical

For D and E series prostaglandin analog screening.

Supplier: MOLECULAR DEVICES

The FLIPR® Membrane Potential Assay Kit provide a fast, simple and reliable, fluorescence-based assay for detecting changes in voltage across the cell membrane and its homogenous fluorescence-based formulations for observation of real-time membrane potential changes associated with ion channel activation and ion transporter proteins.

Supplier: Enzo Life Sciences

LYSO-ID® Red Detection Kit (GFP-CERTIFIED®) is an acidic organelle-selective dye for live cell staining of lysosomes. It is an ideal choice for fluorescence co-localisation imaging with GFP-tagged proteins and providing spectrally pure red signal.

Supplier: MOLECULAR DEVICES

QBT™ Fatty Acid Uptake Assay Kit is a homogeneous assay amenable to high-throughput screening. The kits deliver pre-optimized, fluorescence-based formulations to expedite assay development and screening of fatty acid transporters.

Supplier: Enzo Life Sciences

The LSD1 Fluorimetric Drug Discovery Kit provides human recombinant LSD1 and all reagents for measuring its activity in a sensitive, real-time fluorescent assay.

Supplier: Enzo Life Sciences

The CYTO-ID® Autophagy detection kit provides a rapid, specific and quantitative approach for monitoring autophagy in live cells by fluorescence microscopy and flow cytometry.

Supplier: MOLECULAR DEVICES

Screen and select high value mammalian clones with label-free, in situ detection of IgG secretion with the ClonePix system, and other cell analysers with a green fluorescent channel.

Supplier: Enzo Life Sciences

Designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide. The ADAM17 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide: Mca-PLAQAV-Dpa-RSSSR-NH2. Mca fluorescence is quenched by the Dpa group until cleavage by proteases separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of ADAM17, a potential therapeutic target. The compound GM6001 is also included as a prototypic control inhibitor.

Supplier: MOLECULAR DEVICES

The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. The assay kit is optimised for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis.

Supplier: Enzo Life Sciences

A Rapid, Quantitative and HTS‐compatible Live Cell Cytotoxicity Assay

Supplier: Enzo Life Sciences

For use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins.

Supplier: Enzo Life Sciences

The kit is designed to directly monitor real time reactive oxygen species (ROS) production and Specifically Superoxide in live cells using fluorescence microscopy or flow cytometry.

Supplier: Enzo Life Sciences

A Fluor-de-Lys® fluorescent assay system. The HDAC8 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC8 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The Fluor de Lys® HDAC8 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.

Supplier: Biotium

Stain microtubules in live cells with blue fluorescence.

Supplier: Enzo Life Sciences

For cell cycle results independent of incubation time, temperature, dye and cell concentrations.

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 150,000 MW, and contains a formaldehyde-fixable free-amine group.

Supplier: Enzo Life Sciences

The HDAC2 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC2 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or kinetic assay of the enzyme under varying conditions.

Supplier: Enzo Life Sciences

The kit contains two proprietary dyes suitable for visualizing nucleoli and nuclei simultaneously in living cells.

Supplier: Cayman Chemical

For screening a variety of epigenetic compounds.

Supplier: MOLECULAR DEVICES

CatchPoint™ cAMP Fluorescent Assay Kit measures cAMP levels via a competitive immunoassay format. Cell signaling via G protein-coupled receptors (GPCRs) can be assessed by monitoring the downstream effectors calcium or Cyclic AMP (cAMP). Monitoring levels of cAMP, a second messenger produced in response to activation of adenylate cyclase, is one of the most common ways to screen for agonists and antagonists of GPCRs.

Supplier: Biotium

ExoBrite™ WGA EV staining kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ WGA EV stains bind to molecules in the EV membrane for bright, specific staining, with little background.