You searched for: Cellular Assays

Supplier: Enzo Life Sciences

Ubiquitin Activating Kit for activation of ubiquitin for use in ubiquitinylation experiments. The kit provides the means of generating thioester linked, activated ubiquitin-E1 conjugates, utilising the first step in the ubiquitin cascade, for investigation of ubiquitin activation, subsequent ubiquitin transfer to/interaction with E2 conjugating enzymes, and their use in the ubiquitinylation of E3 ligases and target substrate proteins. Ubiquitin antibody provided allows high sensitivity detection of ubiquitin and ubiquitin-conjugates.

Supplier: Tonbo Biosciences

The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.

Supplier: Enzo Life Sciences

For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays.

Supplier: Biotium

Apoptosis and necrosis are two processes by which cells die. Apoptosis is an active, regulated disassembly of the cell from within. During apoptosis, phosphatidylserine is translocated from the inner to the outer surface of the cell, allowing the dying cell to be engulfed by phagocytic cells. Annexin V is a 35 kD Ca2+-dependent phospholipid binding protein with a high affinity for phosphatidylserine.

Supplier: Biotium

Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.

Supplier: Cayman Chemical

Cholesterol Cell-Based Detection Assay Kit is a 192-well assay for measurement of cholesterol.

Supplier: Enzo Life Sciences

For use with the LEADING LIGHT® Wnt Reporter Cell Line.

Supplier: Enzo Life Sciences

Live cell fluorescent labelling used for a long term cell viability, cytotoxicity, cell adhesion, cell migration, and cell-cell fusion studies.

Supplier: Enzo Life Sciences

Blue IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.

Supplier: Enzo Life Sciences

Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from polyunsaturated fatty acids, are unstable and decompose to form a complex series of compounds. These include reactive aldehydes, of which the most abundant is malondialdehyde (MDA). Therefore, measurement of malondialdehyde is widely used as an indicator of lipid peroxidation. Increased levels of lipid peroxidation products have been associated with a variety of chronic diseases in both humans and model systems.

Supplier: Enzo Life Sciences

Based on the dye cresyl violet and the cathepsin L PheArg peptide recognition sequence, this kit allows the detection of cathepsin L activity in living cells by fluorescence microscopy.

Supplier: Biotium

Cell proliferation dyes diffuse passively into cells and covalently label intracellular proteins, resulting in long term cell labelling. They are non-fluorescent until they are hydrolysed by intracellular esterase's. The dyes then react with intracellular amines forming fluorescent conjugates that are retained in the cell. Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry. With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labelling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analysed immediately after staining. Staining is formaldehyde fixable.

Supplier: Enzo Life Sciences

The kit is designed to directly monitor real time Nitric Oxide production in live cells by fluorescence microscopy using Nitric oxide Detection Reagent (red fluorescent) as the major component.

Supplier: Cayman Chemical

96-Well assay for measurement of p53.

Supplier: Cayman Chemical

A 96-well assay for measurement of PPARδ DNA binding activity.

Supplier: Cayman Chemical

HIF-1α Transcription Factor Assay Kit is a sensitive, non radioactive method for detecting HIF-1α from whole cell lysates.

Supplier: Enzo Life Sciences

The GFP-Certified™ FluoForte™ Calcium Assay kit provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.

Supplier: Enzo Life Sciences

Cellular assay, Fluor-de-Lys®-green substrate, in 50 mM solution in DMSO

Supplier: Biotium

NucView® 488 caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.

Supplier: Biotium

NucView® 488 and MitoView™ 633 kit allows profiling of apoptotic cells simultaneously with changes in mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.

Supplier: Enzo Life Sciences

The LEADING LIGHT® Wnt Reporter Assay Starter Kit is a cell-based luciferase activity test suitable for 96 well plate format.

Supplier: Enzo Life Sciences

Calcium dye

Supplier: Enzo Life Sciences

Based on the dye cresyl violet and the cathepsin B ArgArg peptide recognition sequence, this kit allows the detection of cathepsin B activity in living cells by fluorescence microscopy.

Supplier: AAT BIOQUEST

Lipid peroxidation is the oxidative degradation of cellular lipid by reactive oxygen species (ROS).

Supplier: Enzo Life Sciences

PROTEOSTAT® Aggresome Detection Kit is a robust, quantitative detection of aggresomes relevant to the study of neurodegenerative disease, liver disease and toxicology.

Supplier: Biotium

LysoView™ dyes label lysosomes in live cells, and are available with blue, green, visible red, and far-red fluorescence.

Supplier: Enzo Life Sciences

Cellular assay, Color-de-Lys™ developer concentrate, 300 µl

Supplier: Biotium

Nerve terminal probes are a series of fluorescent cationic styryl dyes developed to follow synaptic activity. These dyes have a lipophilic tail at one end and a hydrophilic cationically charged head group at the other end. The dyes vary in the number of carbons in the lipophilic tail (m) and the number of double bonds linking the two aromatic rings in the dye (n). AM dyes are similar to FM dyes, but contain an additional formaldehyde-fixable amino group in the cationic head.

Supplier: Enzo Life Sciences

The FLUOR DE LYS® HDAC fluorometric activity assay kit is a sensitive and convenient alternative to protocols utilizing radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. It is based on the unique FLUOR DE LYS® (Fluorimetric Histone deAcetylaseLysyl) substrate and developer combination and provides an assay that can be carried out in two simple mixing steps, all on the same 96-well plate. Histone deacetylase inhibitors have shown promise as anti-tumor agents and naturally this has stimulated interest in the screening of compounds for HDAC inhibition.

Supplier: Enzo Life Sciences

The Catalase fluorometric detection kit provides a higher throughput sensitive method for measuring catalase activity in just one hour. This is a homogeneous mix-and-read assay that enables you to monitor multiple time points for a kinetic read. Positive control is provided.