You searched for: Protein Linking and Labeling Systems
Protein linking and labelling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colours, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labelling follows a simple protocol of reaction, separation, and then use.
MS-Compatible magnetic IP kit (Protein A/G), Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Protein A/G) provides mass spectrometry-friendly reagents and an optimised protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS analysis.
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Pierce™ PEGylated BS (bis(sulfosuccinimidyl)suberate) Crosslinkers
Supplier: Thermo Fisher Scientific
Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.
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Long stokes shift dyes, DyLight™, Pierce™
Supplier: Thermo Fisher Scientific
The DyLight™ Long Stokes Shift Dyes are amine-reactive fluorescent dyes that produce long Stokes (LS) shifts of 80 to 145 nm in excitation/emission wavelengths. The DyLight™ LS dyes provide multicolour detection using a single excitation source in applications including flow cytometry, conjugation, DNA-sequencing, microscopy and fluorescence in situ hybridization (FISH). These dyes contain N-hydroxysuccinimide (NHS) esters, the most commonly used reactive group for labeling proteins. NHS esters react with primary amines on proteins and other molecules, forming a stable, covalent amide bond and releasing the NHS group.
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Carbohydrate reactive biotin reagents, HOOK™
Supplier: G-Biosciences
HOOK™ Biotin-Hydrazide and its long spacer arm equivalent, HOOK™ Biotin-LC-Hydrazide, are carbohydrate reactive reagents. HOOK™ carbohydrate reactive biotin reagents react with oxidised carbohydrate side chains. Some biotin reagents do not bind directly to the protein itself, but instead, conjugate to the carbohydrate residues of glycoproteins. The carbohydrate reactive biotin reagents contain hydrazides (-NH-NH₂) as a reactive group. The hydrazide reactions require carbonyl groups, such as aldehydes and ketones, which are formed by oxidative treatment of the carbohydrates. Hydrazides react spontaneously with carbonyl groups, forming a stable hydrazone bond. These reagents are particularly suitable for labelling and studying glycosylated proteins, such as antibodies and receptors.
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Sulfhydryl reactive biotin reagents, HOOK™
Supplier: G-Biosciences
HOOK™-Biotin-PDA, 50 mg
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Protein labelling reagent, iodoTMTzero™
Supplier: Thermo Fisher Scientific
The iodoTMTzero™ label reagent can be used as an isotopic 'light' duplex tag for MS-level protein quantitation.
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HIS Lite™ Cy3 Tris NTA-Ni Complex
Supplier: AAT BIOQUEST
Cy3-Tris NTA compound is used as a sensitive fluorescent probe for detecting polyhistidine-labelled proteins in cells, solution and solid surfaces.
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Helixyte™ iFluor® 647 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 μg DNA/RNA*
Supplier: AAT BIOQUEST
Helixyte™ iFluor® 647 Nucleic Acid labelling Dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.
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ReadiLink™ Rapid Cy3 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)
Supplier: AAT BIOQUEST
Cy3 is one of the most popular fluorescent labelling dyes for preparing orange-red fluorescent bioconjugates.
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ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)
Supplier: AAT BIOQUEST
AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.
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ReadiLink™ xtra Rapid Cy5 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)
Supplier: AAT BIOQUEST
ReadiLink™ xtra rapid antibody labelling kits require essentially only 2 simple mixing steps without a column purification needed.
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ReadiLink™ Rapid mFluor™ Violet 420 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT BIOQUEST
AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.
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ReadiLink™ Rapid mFluor™ Violet 540 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT BIOQUEST
AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.
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iFluor® 750-Wheat Germ Agglutinin (WGA) Conjugate
Supplier: AAT BIOQUEST
iFluor® 750 labelled WGA exhibits a distinct far-red fluorescence characteristic, emitting maximally at 779 nm.
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Buccutite™ Rapid PE Antibody Labelling Kit
Supplier: AAT BIOQUEST
Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PE antibody labelling kits, designed for large-scale production, offer a convenient and efficient method to label antibodies with PE, APC and iFluor® tandem dyes.
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ReadiLink™ xtra Rapid XFD750 Antibody Labelling Kit *BSA-Compatible, XFD750 Same Structure to Alexa Fluor™ 750* (2 Labellings)
Supplier: AAT BIOQUEST
XFD750 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 750 (Alexa Fluor® is the trademark of ThermoFisher).
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Buccutite™ Rapid PE-Cy7 Tandem Antibody Labelling Kit
Supplier: AAT BIOQUEST
Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PE-Cy7 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.
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ReadiLink™ iFluor® 555 FISH Fluorescence Imaging Kit
Supplier: AAT BIOQUEST
The kit provides Taq DNA polymerase enzyme which incorporates iFluor® 555-dUTPs in the target DNA through Polymerase Chain Reaction (PCR).
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ReadiLink™ DIG (Digoxigenin) Nick Translation dsDNA Labeling Kit
Supplier: AAT BIOQUEST
It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.
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ReadiLink™ Biotin Nick Translation dsDNA Labeling Kit
Supplier: AAT BIOQUEST
It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.
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Immobilised reductant column
Supplier: Thermo Fisher Scientific
Pierce™ Immobilised reductant column contains beaded resin to which an thiol-based reducing agent has been immobilised to enable solid-phase reduction of peptide and protein disulphide bonds.
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ReadiLink™ Rapid trFluor™ Eu Antibody Labeling Kit "Microscale Optimised for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT BIOQUEST
Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed.
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Controlled Protein-Protein Crosslinking Kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.
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Pierce™ ABC Staining Kits
Supplier: Thermo Fisher Scientific
ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.
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Buccutite™ Rapid PE-Texas Red Tandem Antibody Labelling Kits (2 Labellings)
Supplier: AAT BIOQUEST
PE-Texas Red is a popular colour used in flow cytometry.
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ReadiLink™ Rapid iFluor® 790 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT BIOQUEST
AAT Bioquest's iFluor® dyes are developed for labelling proteins, in particular, antibodies.
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Buccutite™ Peroxidase (HRP) Antibody Conjugation Kits (2 Labelings)
Supplier: AAT BIOQUEST
Protein-protein conjugations are commonly performed with a bifunctional linker (such as the commonly used SMCC), having different reactivity on each end for linking two different proteins.
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Helixyte™ iFluor® 488 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 µg DNA/RNA*
Supplier: AAT BIOQUEST
Helixyte™ iFluor® 488 Nucleic acid labelling dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.
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Helixyte™ iFluor® 750 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 µg DNA/RNA*
Supplier: AAT BIOQUEST
Helixyte™ iFluor® 750 Nucleic acid labelling dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.
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ReadiLink™ Rapid mFluor™ Red 700 Antibody Labelling Kit 'Microscale Optimised for Labelling 50 µg Antibody Per Reaction' (2 Labellings)
Supplier: AAT BIOQUEST
AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.
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