You searched for: Protein Linking and Labeling Systems

Supplier: Thermo Fisher Scientific

Pierce™ HRP conjugated avidin is used in biotin-based detection methods, including Western blotting or ELISA detection with appropriate enzyme substrates.

Supplier: AAT BIOQUEST

Our Buccutite™crosslinking technology provides the most convenient and effective crosslinking method to link two biomolecules with a high conjugation yield.

Supplier: AAT BIOQUEST

DY547 CEP (DyLight 547 phosphoramidite) is an excellent replacement to the commonly used Cy3 phosphoramidite.

Supplier: Thermo Fisher Scientific

Pierce™ Fluorescence Biotin Quantitation Kit requires only 10 μl of sample to accurately measure the biotinylation level of labeled antibodies and other biomolecules.

Supplier: AAT BIOQUEST

Protein-protein conjugations are commonly performed with a bifunctional linker such as SMCC.

Supplier: AAT BIOQUEST

AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications.

Supplier: AAT BIOQUEST

ReadiLink™ xtra rapid antibody labelling kits require essentially only 2 simple mixing steps without a column purification needed.

Supplier: AAT BIOQUEST

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with XFD dyes (equivalent to Alexa Fluor®).

Supplier: AAT BIOQUEST

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with XFD dyes (equivalent to Alexa Fluor®).

Supplier: AAT BIOQUEST

ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with our superior iFluor® dyes.

Supplier: AAT BIOQUEST

Optimised for labelling 100 µg protein. Buccutite™ rapid oligo antibody conjugation kit is optimised for conjugating 100 µg protein with an amino-modified oligo.

Supplier: AAT BIOQUEST

XFD532, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 532, a trademark of ThermoFisher.

Supplier: AAT BIOQUEST

XFD555, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 555, a trademark of ThermoFisher.

Supplier: AAT BIOQUEST

The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.

Supplier: AAT BIOQUEST

mFluor™ Violet 540 labelled WGA is well-excited by the violet laser, emitting a bright green fluorescence at 535 nm.

Supplier: AAT BIOQUEST

mFluor™ Violet 500 labelled WGA is well-excited by the violet laser, emitting a bright green fluorescence at 501 nm.

Supplier: AAT BIOQUEST

mFluor™ Violet 450 labelled WGA is well-excited by the violet laser, emitting a bright blue fluorescence at 445 nm.

Supplier: Thermo Fisher Scientific

Pierce™ azide-labeled sugars provide a highly specific approach for studying glycoproteins through in vivo metabolic labeling and chemoselective ligation.

Supplier: AAT BIOQUEST

iFluor® 532 WGA conjugate, like Alexa Fluor® 532 WGA conjugate, retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.

Supplier: Thermo Fisher Scientific

Carboxyl groups, in the form of carboxy termini, aspartate residues or glutamate residues, can be targeted for biotin labeling using amine-derivatised biotin molecules. This reaction is mediated by a class of crosslinkers known as carbodiimides and results in the formation of an amide bond. The reaction with EDC, the most common carbodiimide crosslinker, is generally performed in an MES buffer at pH 4,5 to 5 and requires just minutes to complete.

Supplier: AAT BIOQUEST

iFluor® 680 WGA conjugate, like Alexa Fluor® 680 WGA conjugate, retains its ability to bind to sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.

Supplier: AAT BIOQUEST

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PerCP antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PerCP, APC, PE and iFluor® tandem dyes.

Supplier: AAT BIOQUEST

Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ Rapid PE-Cy5 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.

Supplier: AAT BIOQUEST

iFluor® 594 PSA kit is a much superior replacement for Alexa Fluor 594 tyramide-based kit or other spectrally similar fluorescent tyramide or TSA kits.

Supplier: Thermo Fisher Scientific

Photoactivatable Biotin, Biotin-LC-ASA , Psoralen-PEG3-Biotin and TFPA-PEG3-Biotin each contain a photoactivatable group. When exposed to UV light, they become activated and insert non specifically into nearby molecules. These reagents may be used to label proteins and peptides, but they are also useful in labeling DNA, RNA and other molecules that do not contain any readily reactive functional groups. Psoralen-PEG3-Biotin contains a reactive psoralen group and is
designed to efficiently label nucleic acids. The psoralen group intercalates into the helix of DNA, allowing it to label efficiently and selectively. The psoralen can also stack along with the bases of single-stranded DNA or RNA increasing labeling efficiency and selectivity.

Supplier: Thermo Fisher Scientific

The NHS-Azide and NHS-Phosphine Reagents are amine-reactive compounds for derivatising primary amines of proteins or amine-coated polymer surfaces. Once a protein or surface is azide- or phosphine-labelled, the two components are mixed for effective and stable conjugation. Phosphine groups react with azides via a Staudinger reaction to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond. Because phosphines and azides are absent from biological systems, there is minimal background labelling of macromolecules found in cells or lysates.

Supplier: AAT BIOQUEST

The iFluor® 700 conjugate of WGA possesses a bright, far-red fluorescence characteristic of the iFluor® 700 dye (Ex/Em = 690/713 nm).

Supplier: Thermo Fisher Scientific

The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Protein A/G) provides mass spectrometry-friendly reagents and an optimised protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS analysis.

Supplier: Thermo Fisher Scientific

Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used crosslinkers for these applications.

Supplier: Thermo Fisher Scientific

The Thermo Scientific™ EZ-Link™ Plus Activated Peroxidase is an amine-reactive horseradish peroxidase (HRP) that provides higher conjugate yields (>95%) than glutaraldehyde chemistry.